乙型肝炎病毒基因型与拉米夫定疗效关系的研究

To study the relationship between hapatitis B virus genotypes and effect of lamivudine therapy on chronic hepatitis B.Plasma samples of 123 patients with chronic hepatitis B were detected and compared by PCR, Microplate, Hybridization-ELISA.All the patients, 93 patients with genotype B and 30 patients with genotype C, received lamivudine therapy for more than one year.Liver function, viral markers and YMDD mutation were detected one year after treatment.The results showed in B genotype, the recovery rate of ALT was 92.47%, the negative conversion rate of HBeAg was 27.96%, the negative conversion rate of HBV DNA was 82.80%, the antiviral response was 89.25% showing a significant difference as compared with genotype C (P<0.05 or P<0.01) .The emergence rate of YMDD mutation in genotype B was significantly lower than that in genotype C (9.68% and 26.67%, P<0.05) .The antiviral response of lamivudine in HBV genotype B is significantly higher than that in genotype C, and the emergence rate of YMDD mutation in genotype B was significantly lower than that in genotype C.HBV genotypes may be an important determinant of lamivudine therapy of chronic hepatitis B.