顺铂诱导的肝癌细胞凋亡与Fas表达的临床研究

To investigate the relationship between Fas and the apoptosis in hepatocellular carcinoma cells induced by cisplatin. Thirty patients with hepatocellular carcinoma were divided into 2 groups. Sixteen patients received intravenous cisplatin (CDDP) 30mg/day for 4 days preoperatively. whereas the other 14 did not receive any chemotherapy or radiotherapy, and served as controls. Hepatocellular carcinoma cell's apoptosis rate (APO) was examined by flow cytometry (FCM) . The expression of Fas and p53 were examined by immunohistochemical staining. Apoptosis of hepatocellular carcinoma cells increased significantly to 7.72±6.66% in the CDDP-treated group compared with the controls (0.35±0.92%, P<0.01) . Ten patients with Fas protein positive in CDDP-treated group (10/16) were significantly more than 3 positive patients in the controls (3/14, P<0.05) . About p53, there were 5 positive patients in CDDP-treated group and 4 in the controls. In CDDP-treated group, the expression of Fas had no relation with the expression of p53 protein (P>0.05) . Apoptosis in hepatocellular carcinoma cells were enhanced by intravenous infusion of cisplatin. The expression of Fas associated with the apoptosis in hepatocellular carcinoma cells caused by CDDP.