乙肝肝硬化患者HBV前C区和基本核心基因启动子序列分析

The nested polymerase chain reaction (nPCR) was used for amplification of HBV DNA core promoter and PreC in 35 sera form patients with liver cirrhosis in Guangxi, and then HBV DNA nPCR products were sequenced by direct sequencing.The results show that HBV DNA positive rate of 35 patients is 65.7% (23/35) .All patients were infected with mutant.The double mutation (AT at nt1762 and GA at nt 1764) affecting the producing of HBeAg counts for 73.9% (17/23) .And the mutation resulting in stop code at nt 1896 counts for 21.7% (5/23) .Mutation at nt1858 (TC) counts for 21.7%, of which mutation at nt 1856 (CT) was seen in samples 417, 426 and 430.The other mutation such as AG at nt 1752, CG at nt1799, AT at nt1846, TTCCGT at nt 1802-1804 count for 17.4%, 47.8%, 26.1% and 17.4% respectively.The mutation that TG insert between nt 1751-1752, then delete between 1774-1874 was seen in sample 432.The results suggested that HBV core promoter and preC mutations prevalent among patients with liver cirrhosis.These mutations are associated with the development of liver cirrhosis.