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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 6
Jun.  2002
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Article Navigation >  Journal of Clinical Hepatology  > 2002  >  18(6): 334-336

10-23DNA酶体外切割HBV前C/C区mRNA的初步研究

  • Published Date: 2002-06-20
    Abstract
  • To explore the use of synthetic 10-23DNA zyme targeted to PreC/C mRNA as potential therapy for hepatitis B, pCDNA3.1 (+) -Pre C/C vector was established for transcriping target mRNA. Synthesize a 10-23 DNA zyme corresponding to 2031 point of HBV PreC/C mRNA and observe its catalytic activity in vitro. Our results show that synthetic 10-23DNA zyme can efficiently cleave target mRNA in vitro, and its activity is dependent on the concentration of Mg 2+ ion.

     

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