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ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R
Issue 2
Feb.  2000
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乙肝患者外周血单个核细胞中乙肝病毒复制中间产物RNA的检测及临床意义

  • Published Date: 2000-02-20
  • WT5”BZ]We detected hepatitis B virus replicative intermediates RNA in peripheral blood mononuclear cells in patients with hepatitis B virus infection by RT-PCR technology A positive rate of 8 3% was detected for hepatitis B virus replicative intermediates RNA in peripheral blood mononuclear cells in 60 cases of patients with hepatitis B virus infection The positive rate was 13 3% in 30 cases of chronic hepatitis, and it was 6 7% in 15 cases of cirrhosis No one case was detected in 15 cases of acute hepatits This indicated that the replication of hepatitis B virus in peripheral blood mononuclear cells was related to the chronicity of hepatitis B, but was not related to the seriousity of ill [WT5”HZ]

     

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  • []〔1〕DavisonF , AlexanderGJM , AnastassakosC , etal LeucocytehepatitisBvirusDNAinacuteandchronichepatitisBvirusinfection JournalofMedicalVirology , 1987, 2 2∶3 79
    []〔2〕ChomczynskiPandSacchiN Single -stepmethodofRNAisolationbyacidguaridiniumthiocyanate -phenol-chloroformextraction AnalBiochem , 1987, 162∶15 6
    〔3〕分子克隆实验指南 第 2版。 1992 , 681
    〔4〕PasqunelliC HBVDNAinperipheralmononuclearbloodcells:Relation shipwithviralreplication Abstracton 1988meetingonmolecularbiologyofHBV , ColdspringHarbor , 1988, P118
    []〔5〕刘克洲 , 陈慰浙 , 何文南 , 等。血清和肝组织内HBV复制状态与肝脏病变活动性关系的研究。浙江医科大学学报 , 1993 , 2 2 ( 6) ∶2 64
    〔6〕MoraledaG , BartolomeJ, MartinezMG , etal InfluenceofhepatitisdeltavirusreplicationinthepresenceofhepatitisBvirusDNAinperipheralbloodmononuclercells Hepatology, 1990 , 12∶12 90〔7〕Ferayc , ZignegoAl, SamuelD , etal PersistenthepatitsBvirusinfectionofmononuclearbloodcellswithoutconcomitantliverinfection Transplantation , 1990 , 49∶115 5
    []〔8〕HarrisonT HepatitisBvirusRNAinperipheralbloodleakocytes:abriefreview JMedVirol, 1990 , 3 1∶3 3
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      沈阳化工大学材料科学与工程学院 沈阳 110142

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