Expression of Grb2,MMP- 3,and MMP- 9 in cholangiocarcinoma and its significance
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摘要:
目的探讨生长因子受体结合蛋白(Grb)2、基质金属蛋白酶(MMP)3和MMP-9在胆管癌组织中的表达及其意义。方法收集华中科技大学同济医学院附属荆州医院手术切除的47例胆管癌标本,采用免疫组化法检测胆管癌组织及相应正常胆管组织中Grb2、MMP-3和MMP-9的表达情况,并分析Grb2表达与临床病理的关系,以及Grb2表达量与MMP-3、MMP-9表达量之间的关系。计量资料采用t检验,Grb2和MMP-3、MMP-9表达量的相关性分析采用多元线性回归分析。结果 Grb2在胆管癌组织中的表达要明显高于正常胆管组织(t=5.935,P<0.001);Grb2在胆管癌和相应正常胆管组织的表达量与年龄、性别、分化水平无明显相关;在发生淋巴结或者远处转移的胆管癌组织中,Grb2表达量比不发生转移的胆管癌组织要高(t=3.882,P=0.003)。Grb2的表达量与MMP-3、MMP-9的表达量分别呈正相关(r2值分别为0.3667、0.5133,P值分别为0.018、0.007)。结论 Grb2在胆管癌组织的表达量要高于相应正常胆管组织,且Grb2的表达量与肿瘤的侵袭转移能力密切相关;进一...
Abstract:Objective To investigate the expression of growth factor receptor- bound protein- 2( Grb2),matrix metalloproteinase- 3( MMP- 3),and MMP- 9 in cholangiocarcinoma and its significance. Methods The expression of Grb2,MMP- 3,and MMP- 9 in cholangiocarcinoma tissues of 47 cases and normal tissues was measured using immunohistochemistry,and the correlations of Grb2 expression with clinical pathology and MMP- 3 and MMP- 9 expression were analyzed. Comparison of continuous data was made using t test,and the correlation of Grb2 expression with MMP- 3 and MMP- 9 expression was analyzed using the multivariate linear regression model. Results The expression of Grb2 in cholangiocarcinoma tissues was significantly higher than that in normal bile duct tissues( t = 5. 935,P < 0. 001);the expression of Grb2 in cholangiocarcinoma tissues and normal bile duct tissues showed no significant correlation with age,sex,and differentiation level; the expression of Grb2 in cholangiocarcinoma tissues with lymph node or distant metastasis was significantly higher than that in cholangiocarcinoma tissues without metastasis( t = 3. 882,P = 0. 003). The expression of Grb2 was positively correlated with the expression of MMP- 3 and MMP- 9( r2= 0. 3667,P = 0. 018; r2= 0. 5133,P = 0. 007). Conclusion The expression of Grb2 in cholangiocarcinoma tissues is higher than that in normal bile duct tissues,and it is closely related to the invasion and metastasis of carcinoma. Further study shows that the expression of Grb2 is positively correlated with the expression of MMP- 3 and MMP- 9.
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