中文English
ISSN 1001-5256 (Print)
ISSN 2097-3497 (Online)
CN 22-1108/R

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

滤泡性辅助性T细胞与HCV感染的相关性

王宇 马志远 郭永红 何瑜 张颖 谢玉梅 马力 周云 贾战生

引用本文:
Citation:

滤泡性辅助性T细胞与HCV感染的相关性

基金项目: 

国家自然科学基金项目(81170389); 

详细信息
  • 中图分类号: R512.63

Investigation of CD4+CXCR5+ follicular helper T cell frequency and expression of related functional molecules in peripheral blood of hepatitis C virus patients

Research funding: 

 

  • 摘要:

    目的检测慢性HCV感染者外周血CD4+T细胞中滤泡性辅助性T细胞(Tfh)所占比例,ICOS及负性调控因子PD-1、Tim-3在Tfh细胞上的表达。方法以31例HCV感染者、9例HCV感染自愈者(SR-HCV患者)及12例健康志愿者作为研究对象,应用流式细胞术分析外周血中CD4+CXCR5+Tfh细胞在CD4+T细胞中所占比率,同时检测其ICOS、PD-1及Tim-3分子表达,通过酶联免疫吸附法测定血浆中白细胞介素(IL)21及HCV抗体水平,实时定量PCR检测HCV RNA滴度;多组数据比较采用单因素方差分析,双变量之间相关性采用Spearman秩相关分析。结果与健康对照组相比,HCV感染者及自愈者外周血CD4+T细胞比率升高(P<0.05);HCV感染者CD4+CXCR5+Tfh细胞比率高于自愈及健康对照(P<0.05);HCV感染及自愈者CD4+CX-CR5+Tfh细胞ICOS、PD-1、Tim-3显著高于健康对照(P<0.05);血清il-21水平hcv感染者与健康对照无统计学差异(p>0.05);HCV感染者HCV RNA载量与Tfh细胞比例呈负相关。...

     

  • [1]Rehermann B.Chronic infections with hepatotropic viruses:mecha-nisms of impairment of cellular immune responses[J].Semin LiverDis, 2007, 27 (2) :152-160.
    [2] Huang XJ, Lv X.Progress in hepatitis C vaceine[J].Int J Virol, 2011, 18 (1) :29-32. (in Chinese) 黄小军, 吕欣.丙型肝炎疫苗研究进展[J].国际病毒学杂志, 2011, 18 (1) :29-32.
    [3] Feng J, Hu X, Guo H, et al.Patients with chronic hepatitis C expressa high percentage of CD4 (+) CXCR5 (+) T follicular helper cells[J].J Gastroenterol, 2012, 47 (9) :1048-1056.
    [4]Rodríguez Pinilla SM, Roncador G, Rodríguez-Peralto JL, et al.Pri-mary cutaneous CD4+small/medium-sized pleomorphic t-cell lym-phoma expresses follicular T-cell markers[J].Am J Surg Pathol, 2009, 33 (1) :81-90.
    [5]Simpson N, Gatenby PA, Wilson A, et al.Expansion of circulating Tcells resembling follicular helper T cells is a fixed phenotype that iden-tifies a subset of severe systemic lupus erythematosus[J].ArthritisRheum, 2010, 62 (1) :234-244.
    [6]Rodríguez-Pinilla SM, Atienza L, Murillo C, et al.Peripheral T-cell lymphoma with follicular T-cell markers[J].Am J SurgPathol, 2008, 32 (12) :1787-1799.
    [7]Feng JY, Lu L, Hua C, et al.High frequency of CD4+CXCR5+TFHcells in patients with immuneactive chronic hepatitis B[J].PLoS ONE, 2011, 6 (7) :e21698.
    [8]Moreno C, Deltenre P.Management of chronic hepatitis C in 2012 inBelgium[J].Rev Med Brux, 2012, 33 (4) :223-227.
    [9]Kim CH, Rott LS, Clark-Lewis I, et al.Subspecialization of CX-CR5+T Cells:B helper activity isfocused in a germinal center–localized subset of CXCR5+T Cells[J].J Exp Med, 2001, 193 (12) :1373-1381.
    [10]Feng JY, Lu L, Hua C, et al.High frequency of CD4+CXCR5+TFHcells in patients with immuneactive chronic hepatitis B[J].PLoS ONE, 2011, 6 (7) :e21698.
    [11]Linterman MA, Vinuesa CG.T Follicular Helper Cells During Im-munity and Tolerance[J].Prog Mol Biol Transl Sci, 2010, 92:207-248.
    [12]Chen M, Guo Z, Ju W, et al.The development and function offollicular helper T cells in immune responses[J].Cell Mol Immu-nol, 2012, 9 (5) :375-379.
    [13]Deenick EK, Ma CS.The regulation and role of T follicular helpercells in immunity[J].Immunology, 2011, 134 (4) :361-367.
    [14]Wang Y, Zhang Y, Jia ZS.Recent treatment advances for hepatitisC virus-related mixed cryoglobulinemia[J].J Clini Hepatol, 2012, 28 (8) :628-632 (in Chinese) 王宇, 张颖, 贾战生.丙型肝炎病毒相关性混合型冷球蛋白血症的治疗进展[J].临床肝胆病杂志, 2012, 28 (8) :628-632.
  • 加载中
计量
  • 文章访问数:  2740
  • HTML全文浏览量:  8
  • PDF下载量:  614
  • 被引次数: 0
出版历程
  • 出版日期:  2013-05-20
  • 分享
  • 用微信扫码二维码

    分享至好友和朋友圈

目录

    /

    返回文章
    返回