PDF下载 ( 1238 KB)
Preliminary effect of autologous bone marrow stem cells transplantation for liver failure
-
摘要: 目的观察自体骨髓干细胞移植治疗肝衰竭的初步疗效。方法 12例经常规内科综合治疗无明显效果的肝衰竭患者。在无菌条件下采集骨髓100～200ml,通过密度梯度离心分离纯化出骨髓干细胞,通过介入的方法经肝动脉注射入肝内,静脉输注肝细胞生长素1～2周。观察移植后1、2、3和6月患者症状、体征、肝脏生化和凝血酶原时间等的变化。结果 9例肝衰竭患者的症状、体征改善,白蛋白升高、胆红素降低,肝脏功能改善,所有患者未见明显副作用。结论自体骨髓干细胞移植治疗肝衰竭安全有效。Abstract: Objective To evaluate the preliminary effect of autologous bone marrow stem cells transplantation for liver failure.Methods Twelve patients with liver failure were studied.100-200 ml bone marrow from each patient was harvested in aseptic condition.After isolation and purification by density gradient centrifugation, the stem cells were obtained and transplanted into the liver via hepatic artery.All patients were injected hepatic growth factor for 1-2 weeks.Clinical symptoms and signs and liver function and prothrombin time were checked after l, 2, 3 and 6 months of transplantation respectively.Results After transplantation, clinical symptoms and signs and laboratory data of 9 patients were improved and levels of albumin were increased significantly.Complications and serious side effects were not observed.Conclusion The treatment for liver failure by transplantation of autologous bone marrow stem cells is safe and effective.
-
Key words:
- bone marrow transplantation /
- stem cell transplantation /
- transplantation /
- autologous
-
[1] 中华医学会感染病学分会肝衰竭与人工肝学组.肝衰竭诊疗指南[J].中华传染病杂志, 2006, 24 (6) ∶422-425. [2]Sakaida I, Terai S, Nishina H, et al.Development of cell therapy using autologous bone marrow cells for liver cirrhosis[J].Med Mol Morphol, 2005, 38 (4) ∶197-202. [3]Zhan YT, Wei L, Chen HS, et al.Differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment[J].ZhonghuaGanzangbing Zazhi, 2003, 11 (11) ∶673-675. [4]Fang B, Shi M, Liao L, et al.Systemic infusion of FLK1 (+) mesenchymal stem cells ameliorate carbon tetrachloride-induced liverfibrosis in mice[J].Transplantation, 2004, 78 (1) ∶83-88. [5]Yannaki E, Athanasiou E, Xagorari A, et al.G-CSF-primedhematopoietic stem cells or G-CSF per se accelerate recovery andimprove survival after liver injury, predominantly by promotingendogenous repair programs[J].Exp Hematol, 2005, 33 (1) ∶108-119. [6] 高志良, 彭亮, 崇雨田, 等.骨髓间充质干细胞自体移植治疗慢性肝衰竭的疗效研究[J].现代消化及介入诊疗, 2007, 12 (S) ∶2327. [7]姚鹏, 王帅, 胡大荣, 等.肝动脉自体骨髓干细胞移植治疗失代偿期肝硬化30例[J].世界华人消化杂志, 2005, 13 (13) ∶1639-1640. [8]夏凌辉, 方峻, 游泳, 等.造血干细胞移植后肝静脉闭塞病的临床观察[J].临床内科杂志, 2007, 24 (2) ∶98-100. [9]Lorenzini S, Andreone P.Stem cell therapy for human liver cirrhosis:A cautious analysis of the results[J].Stem Cells, 2007, 25 (9) ∶2383-2384. -
本文二维码
计量
- 文章访问数: 2873
- HTML全文浏览量: 16
- PDF下载量: 719
- 被引次数: 0
下载:
