肝炎肝硬化患者骨髓TPO、GM-CSF表达与外周血细胞的关系

王顺达; 党双锁; 程延安; 高宁; 张欣; 贾晓黎

肝炎肝硬化患者骨髓TPO、GM-CSF表达与外周血细胞的关系

西安交通大学医学院第二附属医院感染科

基金项目:

国家自然科学基金资助项目(30670961)；

详细信息

中图分类号: R575.2
计量
- 文章访问数: 3855
- HTML全文浏览量: 17
- PDF下载量: 719
- 被引次数: 0
出版历程
- 出版日期: 2010-05-20

The expression of TPO and GM - CSF in the marrow fluid and the relations with peripheral blood cells of liver cirrhosis patients

Research funding:

摘要

摘要: 目的探讨骨髓内环境造血因子血小板生成素（TPO）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）在肝炎肝硬化患者骨髓液中的表达及其与外周血细胞的关系。方法选取31例肝炎肝硬化患者和15例健康对照组,晨起空腹采血、检测血常规、肝脏功能、肝炎病毒标志物,并行骨髓穿刺术,取骨髓液、离心分离,用双抗体夹心ELISA法检测骨髓液中TPO、GM-CSF浓度。结果肝炎肝硬化患者骨髓液TPO浓度与外周血血小板计数呈负相关（r=-0.496,P<0.05）。骨髓液TPO的浓度在肝炎肝硬化组和对照组分别为（90.756±30.92）pg/ml、（118.414±49.232）pg/ml,二者之间有差异,但差异无统计学意义。肝脏功能按照Child-Pugh分为A、B、C级,分别与对照组比较,发现随肝脏损害加重,TPO浓度呈下降趋势,但差异无统计学意义。GM-CSF的浓度在肝硬化组和对照组之间差异无统计学意义。结论肝炎肝硬化患者骨髓内环境TPO浓度降低可能是外周血血小板计数减少的原因之一。肝脏功能状态及GM-CSF与外周血细胞计数变化的关系还有待进一步研究。
- 血小板生成素 /
- 粒细胞-巨噬细胞集落刺激因子 /
- 肝硬化 /
- 血细胞计数
Abstract: Objective To investigate the expression of TPO and GM-CSF in the marrow fluid and the relations with pe-ripheral blood cells of hepatitis cirrhosis patients.Methods Thirty one liver cirrhotic patients and 15 healthy controls were included in the study.Routine blood tests, liver function tests and hepatitis virus markers were analyzed.TPO and GM-CSF concentration of liver cirrhosis patients and healthy control group were detected by enzyme linked immunosorbent assay (ELISA) .Results TPO concentration had negatively correlated with the platelet count (r =-0.496, P < 0.05) .There was no statistically significant difference between the TPO concentration in cirrhotic group (90.756 ± 30.92) pg/ml and the control group (118.414 ± 49.232) pg/ml.Liver cirrhosis patients were classified into three groups according to liver Child-Pugh grade.However, there was no difference between the Child-Pugh grade A, B and C groups, and the control group.There was no significant difference in CM-CSF concentration between the cirrhotic and the control group.Conclu-sion The marrow fluid TPO concentration decline in hepatitis cirrhotic patients might affect the platelet concentration.The relationship between the liver function, CM-CSF and different peripheral blood cells needs further studies.
- thrambopoietin /
- granulocyte-macrophage colong-stimulating factor /
- liver cirrhosis /
- blood cell count

HTML全文

参考文献(11)

[1]Raquel R, Bruno S, Ignacio H, et al.The role of thrombopoietin inthe thrombocytopenia of patients with liver cirrhosis[J].Am J Gas-troenterol, 2005, 100 (6) ∶1311-1316.

[2]党双锁.肝硬化失代偿期外周血细胞减少成因机制[J].世界华人消化杂志, 2007, 15 (27) ∶2861-2864.

[3]中华医学会.病毒性肝炎防治方案[J].中华预防医学杂志, 2000, 1 (1) ∶44-52.

[4]隋杰, 肖灿鹏.人血小板生成素的研究进展[J].生物技术通讯, 2002, 13 (1) ∶76-82.

[5]Shimabukuro R, Kawanaka H, Tomikawa M, et al.Effect of throm-bopoietin on platelet counts and liver regeneration after partial hepa-tectomy in a rat model[J].Surg Today, 2009, 39 (2) ∶1054-1059.

[6]de Sauvage FJ, Carver-Moore K, Luoh SM, et al.Physiologicalregulation of early and late stages of megakaryocytopoiesis by throm-bopoietin[J].J Exp Med, 1996, 183 (2) ∶651-656.

[7]Martin TG 3rd, Somberg KA, Meng YG, et al.Thrombopoietin lev-els in patients with cirrhosis before and after orthotopic liver trans-plantation[J].Ann Intern Med, 1997, 127 (4) ∶285-288.

[8]Weksler BB.The pathophysiology of thrombocytopenia in hepatitisC virus infection and chronic liver disease[J].Aliment PharmacolTher, 2007, 26 (1) ∶13-19.

[9]Metcalf D.Hematopoietic cytokines[J].Blood, 2008, 111 (2) ∶485-491.

[10]Berghams T, Paesmans M, Lafitte JJ, et al.Therapeutic use ofgranulocyte and granulocyte-macrophage colony-stimulating fac-tors in febrile neutropenic cancer patients-A systematic review ofthe literature with meta-analysis[J].Support Care Cancer, 2002, 10 (3) ∶181-188.

[11]Kubotaa, Okamura S, Omori F, et al.High serum levers of granulo-cyte-macrophage colony-stimulating factor in patients with liver-cirrhosis and granulocytopenia[J].Clin Lab Haematol, 1995, 17 (1) ∶61-63.

施引文献

资源附件(0)

访问统计