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Ganfukang's effect on transforming growth factor β1 and laminin of experimental liver fibrosis rats
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摘要: 研究复方中药肝复康对实验性肝纤维化大鼠肝组织的转化生长因子 β（TGF—β1）及层粘连蛋白 （LN）表达的影响 ,探讨其治疗机制。采用 10 %的四氯化碳皮下注射制备肝纤维化模型 ,于造模 6 0天后给予肝复康治疗 6 0天 ,以秋水仙碱及肝脾康为对照药。通过光镜及电镜观察肝组织病理变化 ,检测肝组织羟脯氨酸含量 ,免疫组化法检测肝组织TGF - β1及LN表达。经肝复康治疗后 ,组织学显示肝纤维化程度明显改善 ,肝组织羟脯氨酸明显降低 ,肝组织TGF - β1阳性染色细胞数及LN阳性染色面积明显减少 （P <0 0 1）。肝复康对肝纤维化有明确的治疗作用。其部分机制为减少细胞因子TGF - β1的表达 ,减少LN的表达 ,减轻肝窦毛细血管化Abstract: To study Ganfukang's effect on transforming growth factor β1 (TGF β1) and laminin (LN) of experimental liver fibrosis rats.The hepatic fibrosis in rat were induced by CCl4.and then treated with Ganfukang, using colchicines and Ganpikang as control drugs.Afrer treatment, the hepatic hydroxyproline (Hyp) content were measured, and histopathological changes were observed by optical microscopy and electromicrocopy, TGF β1 and LN were observed with immunohistochemistry stain method.In Ganfukang group, Hyp and the degree of liver fibrosis were markedly reduced respectively compared with those of control group, and the content of TGF β1 and LN decreased.Ganfukang can reduce experimental liver fibrosis, it partial mechanism is that reducing the expression of TGF β1and LN, and alleviate sinusoid capillarization.
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Key words:
- experimental liver fibrosis /
- Ganfukang /
- transforming growth factor β1 /
- laminin
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[1]周永兴.现代肝硬化诊断治疗学[M ].北京:人民军医出版社, 2002∶13. [2]焦柏忠, 刘晓晶, 吕美丽, 等, 肝脾康胶囊药理作用研究[J].中草药, 1999, 30 (12) ∶926～929. [3] WeinerFR , GrambroneMA , CzjaMJ, etal.Ito-cellgeneexpressionandcollagenregulation[J].Hepatology, 1990, 11 (1) ∶111～118. [4] TanikawaK .Serummarkerforhepaticfibrosisandrelatedliverpathology[J].PatholResPract, 1994, 190∶960～968. -
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