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The mutations in the core promoter and the precore gene of hepatitis B virus in patients with chronic hepatitis B positive for anti-HBe.
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摘要: 研究抗 -HBe阳性乙型肝炎病毒C基因启动子 （CP）和前C基因变异。用基因序列分析检测基因变异。（1）抗 -HBe阳性患者的前C终止变异 （nt1896G→A）的发生率显著高于抗 -HBe阴性组 （P <0 0 0 1） ;而CP双变异（nt176 2A→T和 176 4G→A）则在两组中无明显差异。 （2 ）同抗 -HBe阳性慢性乙肝组比较 ,抗 -HBe阳性重型乙肝患者的前C终止变异和CP双变异发生率无明显差异 ,而CPnt175 2A→G变异发生率则显著增高 （P <0 0 5 ）。前C终止变异与抗 -HBe阳性乙型肝炎密切相关 ;而CPnt175 2变异则可能与抗 -HBe阳性重型乙型肝炎发病有关
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关键词:
- 乙型肝炎病毒C基因启动子 /
- 前C基因变异 /
- 聚合酶链反应
Abstract: To study the mutations in the core promoter (CP) and the precore gene of hepatitis B virus in patients with chronic hepatitis B (CH) positive for anti-HBe.CP and precore gene were sequenced directly from sera, after amplication by the polymerase chain reaction. Precore mutation in nt 1896 (G→A) were observed in 21 of 34 of patients with anti-HBe positive CH and in 8 of 55 of patients with anti-HBe negative CH, the difference of which was significant.There was no significant difference in the prevalence of the double mutations (A→T at nt 1762 and G→A at nt 1764) between two groups.The mutation in nt 1752 (A→G) were observed in 8 of 10 of patients with chronic severe hepatitis B positive for anti-HBe and in 7 of 24 of patients with anti-HBe positive CH, the difference of which was significant.Precore mutation in nt1896 is related to chronic hepatitis B positive for anti-HBe.The mutation in nt1752 may be associated with severe hepatitis B positive for anti-HBe.-
Key words:
- Hepatitis B /
- Core promoter /
- Precore gene
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