Abstract: To probe into Arg-Gly-Asp-Ser (RGDS) peptide inhibiting hepatic stellate cells synthesizing extracellar matrix stimulated by transforming growth factor beta (TGF β) . Hepatic Stellate cells (HSCs) were isolated from rat liver (male normal Sprague-Dawleyv rats; 550g) and plated onto 24-well uncoated plastic at a density 1×10 6 cells/ml and cultured. 6 groups were random divided which every group was 3 well cells. After cells cultured 72 hours, 100 μl cultured supernatants was respectively sucked from every well. Then, equality culturing medium was immited in the simple cultured cell control group, RGDS 100μg/ml was dropped into RGDS group wells, 100ng/ml TGF β 1 was only infused in simple TGF β 1 stimulating control group, and at one time TGF β 1 infused into other groups, 100μg/ml (final concentration) RGDS was infused in RGDS group and 100μ g/ml RGES in RGES group as well as 100U/ml interferon (IFN) α-2b in IFN group respectively, and they had been incubated together for 24 hours. Since, 100μl supernatants were taken from very well marked separately. The collagen Ⅲ level in the supernatants was detected by enzymelinked immunosorbent assay (ELISA) . The collagen Ⅲ level of RGDS+TGF β 1 group and IFN+TGF β 1 group decreased significantly than of TGF β control group and RGES+TGF β 1 group (P<0.05) . RGDS peptide can inhibit TGF β 1 accelerate hepatic stellate cells synthesizing collagen Ⅲ.