乙肝患者外周血单个核细胞中乙肝病毒复制中间体RNA与乙肝病毒DNA相关性的研究

张凯宇; 蔡艳俊; 王峰; 许昶; 吕美德

乙肝患者外周血单个核细胞中乙肝病毒复制中间体RNA与乙肝病毒DNA相关性的研究

详细信息

中图分类号: R512.62
计量
- 文章访问数: 2214
- HTML全文浏览量: 9
- PDF下载量: 813
- 被引次数: 0
出版历程
- 出版日期: 2001-03-20

Study on Relationship between Detection of Hepatitis B Virus Replicative Intermediates RNA and Hepatitis B Virus DNA in Peripheral Blood Mononuclear Cells in Patients with Hepatitis B Virus Infection

摘要

摘要: 采用逆转录 -多聚酶链反应（RT -PCR）技术检测乙肝患者外周血单个核细胞（PBMCs）乙肝病毒复制中间体RNA（HBVRNA） ,6 0例乙肝患者 5例PBMCs中HBVRNA阳性 ,2 6例PBMCs中HBVDNA阳性 ,2 7例血清HBVDNA阳性 ;PBMCsHBVDNA阳性患者中有 4例PBMCsHBVRNA阳性 ,血清HBVDNA阳性患者中有3例PBMCsHBVRNA阳性 ;17例血清HBeAg阳性患者中有 4例PBMCsHBVRNA阳性。血清HBeAg、HBVDNA及PBMCs中HBVDNA阴性患者 ,其PBMCsHBVRNA仍可能阳性 ,其PBMCs可成为乙肝患者肝病复发和传播的来源
- 外周血单个核细胞 /
- 乙肝病毒 /
- 乙肝病毒复制中间体RNA /
- 多聚酶链反应
Abstract: We detected HBV replicative intermediates RNA in PBMCs in patients with HBV infection by RT PCR technology, meanwhile detected HBV DNA in PBMCs and serum by PCR technology.Five cases were detected for HBV RNA in PBMCs in 60cases of patients with HBV infection.HBV DNA were positive in both PBMCs in 26 cases and serum in 27 cases.In 26 cases whose HBV DNA in PBMCs were positive , 4 cases of HBV RNA in PBMCs were positive, In 27 cases whose HBV DNA in serum were positive, 3 cases of HBV RNA in PBMCs were positive.HBeAg was also detected in serum, in 17 cases whose HBeAg were positive, 4 cases of HBV RNA in PBMCs were positive.Though HBeAg and HBV DNA in serum, HBV DNA in PBMCs were negative in patients with HBV infection, HBV RNA in PBMCs may be positive, HBV in PBMCs could become the resource of recidimation and transmission.

HTML全文

参考文献(9)

[1]DejeanA , lugassyC , ZafraniS , etal.DetectionofhepatitisBvirusDNAinpancreas , kidneyandskinoftwohumancarriersofvirus[J] .JournalofGeneralvirology , 1984, 6 5∶6 5 1.

[][2 ]Romet lemonneJ L , MclaneMF , ElfassiE , etal.HepatitisBvirusinfectioninculturedhumanlymphoblastoidcells[J] .Science , 1983 , 2 2 1∶6 6 7.

[3]DavisonF , AlexanderGJM , AnastassakosC , etal.Leucocytehepati tisBvirusDNAinacuteandchronichepatitisBvirusinfection[J] .JournalofMedicalvirology , 1987, 2 2∶379.

[4]黄育清 , 白敬羽 , 葛其童 , 等乙型病毒性肝炎患者外周血中单个核细胞及血清内HBVDNA的检测及其意义 [J] .临床检验杂志 , 1995 , 13 (1) ∶16

[][5 ]病毒性肝炎防治方案 (试行 ) [J] .中华传染病杂志 , 1995 , 13 (4) ∶2 41.

[][6 ]ChomczynskiPandSacchiN .Single stepmethodofRNAisolationbyacidguaridinumthiocyanate phenol chloroformextraction [J] .AnalBiochem , 1987, 16 2∶15 6 .

[7]SuT S , LuiW Y , LinH L , etal.AnalysisofhepatitisBvirustran scriptsininfectedhumanlivers[J] .hepatology , 1989, 19 (2 ) ∶180 .

[8]ShenHD , ChooKB , LeeSD , etal.HepatitisBvirusDNAinLeuko cytesofpatientswithhepatitisBvirus associatedliverdisease[J] .JMedVirol, 1986 , 18∶2 0 1.

[9]MillerRH , RobinsonWS .IntegratedhepatitisBvirusDNAse quencesspecifyingthemajorvirolcorepolypeptideandmethylatedinPLT/PRF/5cells[J] .ProcNatlAcadSciUSA , 1883 , 80∶2 5 34.

施引文献

资源附件(0)

访问统计