肝癌类器官的研究进展

赵丽; 郭梓琪; 杨勇; 杨宏新

doi:10.12449/JCH240730

肝癌类器官的研究进展

DOI: 10.12449/JCH240730

赵丽¹,
郭梓琪¹,
杨勇²,
杨宏新^1, , ,

1.
内蒙古医科大学细胞生物学教研室，呼和浩特 010110
2.
内蒙古自治区人民医院腹部肿瘤外科，呼和浩特 010017

基金项目:

国家自然科学基金 (81960772);

内蒙古医科大学重点项目 (YKD2022ZD016);

内蒙古自治区自然科学基金 (2019MS08087);

内蒙古医科大学肿瘤类器官基因编辑转化医学科技创新团队项目

利益冲突声明：本文不存在任何利益冲突。

作者贡献声明：赵丽负责选题，总结文献，撰写文章；郭梓琪负责查阅文献，修改论文；杨勇、杨宏新负责校正并最后定稿。

详细信息

通信作者:
杨宏新， hongxinydiy@sina.com （ORCID： 0000-0002-5097-3260）

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出版历程
- 收稿日期: 2023-11-18
- 录用日期: 2024-02-27
- 出版日期: 2024-07-25

Research advances in liver cancer organoids

Li ZHAO¹,
Ziqi GUO¹,
Yong YANG²,
Hongxin YANG^{1
, ,
,}

1.
Department of Cell Biology，Inner Mongolia Medical University，Hohhot 010110，China
2.
Department of Abdominal Oncology，Inner Mongolia Autonomous Region People’s Hospital，Hohhot 010017，China

Research funding:

National Natural Science Foundation of China (81960772);

Inner Mongolia Medical University Key Project (YKD2022ZD016);

Natural Science Foundation of Inner Mongolia Autonomous Region (2019MS08087);

Inner Mongolia Medical University Tumor Organoid Gene Editing Translational Medicine Science and Technology Innovation Team Project

More Information

Corresponding author: YANG Hongxin， hongxinydiy@sina.com （ORCID： 0000-0002-5097-3260）

摘要

摘要: 类器官是一种由干细胞或恶性肿瘤自我组装形成并用于临床研究的新型疾病模型，类似于体内的组织与器官，且具有部分功能的3D细胞结构。传统的肝癌模型有两种：一是通过诱导构建动物肝癌模型，即体内模型；二是在体外通过相应细胞系进行细胞学实验研究。类器官集合了传统模型的优点，在肿瘤研究方面具有独特的优势。传统模型由于不能真实反映细胞微环境，常导致与临床研究结果不一致，而新型研究模型的出现，为肝癌研究提供了一个新的方向。本文对肝癌类器官的研究进展进行综述，旨在为今后肝癌研究提供全新的视角。
- 肝肿瘤 /
- 类器官 /
- 细胞培养技术
Abstract: Organoids are a novel disease model that is self-assembled from stem cells or malignant tumors and is used in clinical research. They are similar to tissues and organs in the body and have partially functional 3D cell structures. There are two types of traditional models for liver cancer research， i.e.， in vivo models （animal models of liver cancer established by induction） and in vitro cell experiments using corresponding cell lines. Organoids have the advantages of the two types of traditional models and show unique advantages in tumor research. Traditional models cannot fully reflect the microenvironment of cells， which often leads to the inconsistency with clinical research findings， and the emergence of new research models provides a new direction for the research on liver cancer. This article reviews the research advances in liver cancer organoids， in order to provide a new perspective for future research on liver cancer.
- Liver Neoplasms /
- Organoids /
- Cell Culture Techniques

HTML全文

图 1 肝癌类器官的构建过程

注：对ESC或iPSC进行提取和培养，加入所需的细胞因子EGF、HGF、FGF等，载体支架为细胞提供生长环境，在培养皿中进行培养待其长成初步具有形态的类器官。

Figure 1. The construction process of liver cancer organoids

下载: 全尺寸图片幻灯片

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