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高频不可逆电穿孔消融猪胰腺组织的效果观察

幸嵘 杜霁儒 翁原驰 王峰 刘超

引用本文:
Citation:

高频不可逆电穿孔消融猪胰腺组织的效果观察

DOI: 10.12449/JCH240523
基金项目: 

山东省自然科学基金面上项目 (ZR2023MH111)

医学伦理声明本实验中的所有操作均遵守动物福利法规,且已通过上海甲干生物科技有限公司伦理委员会批准,批号:JGLL-20210316002。
利益冲突声明:本文不存在任何利益冲突。
作者贡献声明:幸嵘负责实验设计和操作,资料分析,撰写论文;杜霁儒负责资料分析,撰写论文;翁原驰负责动物实验操作;王峰参与实验设计及修改论文;刘超负责拟定写作思路,指导撰写文章并最后定稿。幸嵘、杜霁儒对本文贡献等同,同为第一作者。
详细信息
    通信作者:

    刘超, liuchao2311@126.com (ORCID: 0009-0002-9203-5800)

Effect of high-frequency irreversible electroporation in the ablation of porcine pancreatic tissue

Research funding: 

Shandong Provincial Natural Science Foundation Project (ZR2023MH111)

More Information
  • 摘要:   目的  探索高频不可逆电穿孔(H-FIRE)消融猪胰腺组织的效果。  方法  采用开腹手术,借助针式电极对12只猪释放电脉冲,按照场强数值小、中、大设置三组参数(1 000 V/cm、1 500 V/cm、2 500 V/cm)进行消融。通过比较各组术后恢复情况、消融面积、组织病理学表现等数据验证H-FIRE消融猪胰腺组织的安全性、有效性。计量资料两组间比较使用配对t检验。  结果  所有实验猪均存活,且获得明确的消融效果,各组组织病理学均提示,消融有效彻底,消融区与正常组织区分界明显,小场强组、中场强组、大场强组平均消融面积分别为(30.96±3.73)mm2、(51.93±25.26)mm2、(108.90±55.23)mm2,大、中场强组消融面积均显著大于小场强组(P值均<0.05),中场强组与大场强组消融面积差异无统计学意义(P>0.05)。  结论  在特定的消融参数下,对猪胰腺进行H-FIRE消融安全、有效。

     

  • 图  1  实验猪行H-FIRE消融术中情况

    注: a,胰腺行H-FIRE消融过程中;b,消融后撤除电极针(箭头所示为消融部位)。

    Figure  1.  Intraoperative situation during the H-FIRE ablation procedure in the experimental pigs

    图  2  实验猪胰腺行H-FIRE消融术后7天病理表现

    注: a~c(小、中、大场强),实线勾画出区域即消融区,其中心表现为片状坏死,周围胰腺组织变性,纤维组织增生,炎细胞浸润(HE染色, ×0.86, ×0.45,×0.31);d~f(小、中、大场强),显示损伤灶内胰腺组织变性坏死及局灶出血改变(HE染色,×20)。

    Figure  2.  Pathological findings at 7th day after H-FIRE ablation

    图  3  各组参数的消融区域面积比较

    Figure  3.  Comparison of the ablation area of each group

    图  4  实验猪胰腺行H-FIRE消融术后7天MRI表现

    注: a,T1W横断面显示主胰管扩张,胰管近端胰头消融区域斑片状低信号,边界清晰;b,T2W横断面显示消融区为不均匀高信号,边界欠清;c,增强扫描显示消融区域内无强化,边界较清晰。箭头所示实线勾画的区域即消融灶。

    Figure  4.  MRI findings at 7th day after H-FIRE ablation

    表  1  实验室检查结果

    Table  1.   Laboratory test results

    指标 术前(n=12) 术后第7天(n=12) t P
    红细胞计数(×1012/L) 6.86±1.09 6.81±1.16 0.11 0.915
    血红蛋白(g/L) 121.00±5.73 119.00±9.31 0.59 0.568
    白细胞计数(×109/L) 15.22±1.40 20.50±2.02 10.63 <0.001
    血小板计数(×109/L) 388.00±109.01 438.67±84.06 1.31 0.216
    血清淀粉酶(U/L) 3 170.67±1 235.67 2 988.00±1 403.83 0.41 0.688
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  • 收稿日期:  2023-08-30
  • 录用日期:  2023-10-27
  • 出版日期:  2024-05-25
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