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CCl4诱导的肝纤维化大鼠模型肝小叶内卵圆细胞总体积与轮廓数密度变化的体视学分析

王川林 刘全明 杨霞 杨正伟 梅小平 彭彬

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CCl4诱导的肝纤维化大鼠模型肝小叶内卵圆细胞总体积与轮廓数密度变化的体视学分析

DOI: 10.12449/JCH240113
基金项目: 

川北医学院2021年度校级科研发展计划项目 (CBY21-QA14)

南充市2020年市校科技战略合作专项 (20SXQT0304)

利益冲突声明:本文不存在任何利益冲突。
作者贡献声明:彭彬、杨正伟、梅小平负责设计研究方案并提供指导性支持;王川林、刘全明、杨霞负责数据收集、整理与分析;王川林、彭彬负责论文撰写与修改。
详细信息
    通信作者:

    彭彬, 340255492@qq.com (ORCID: 0000-0002-6947-2570)

Changes in the total volume and contour density of oval cells in hepatic lobules of rats with carbon tetrachloride-induced hepatic fibrosis: A stereological study

Research funding: 

North Sichuan Medical College 2021 School-Level Scientific Research Development Plan Project (CBY21-QA14);

Nanchong Special Project for Municipal and University Science and Technology Strategic Cooperation in 2020 (20SXQT0304)

More Information
    Corresponding author: PENG Bin, 340255492@qq.com (ORCID: 0000-0002-6947-2570)
  • 摘要:   目的  定量研究CCl4诱导的肝纤维化大鼠肝小叶内卵圆细胞(HOC)总体积和轮廓数密度的变化。  方法  将11只雄性健康SD大鼠随机分为对照组(n=5)、肝纤维化组(n=6),每周2次皮下注射CCl4与橄榄油混悬液,每次3 mL/kg。在肝纤维化造模5周后取材,从每只大鼠肝脏随机抽选5个大小约1 mm3的肝组织块分别制作1张Epon812环氧树脂包埋超薄切片,运用体视学方法结合透射电子显微镜技术,对大鼠肝小叶内的HOC总体积和轮廓数密度进行定量研究,另从每只大鼠剩余肝脏等距随机抽选4个2 mm厚的肝组织块并分别制作2张石蜡包埋的Masson染色切片,按照肝纤维化Metavir分期标准定性评估每只大鼠的肝纤维化程度。计量资料两组间比较采用成组t检验。  结果  体视学定量研究显示,对照组肝小叶内HOC总体积为(15.40±7.63) mm3,肝纤维化组肝小叶内HOC总体积为(146.80±114.00) mm3,与对照组比较,肝纤维化组大鼠肝小叶内HOC总体积显著增加了8.53倍(t=-2.551,P=0.031);对照组肝小叶内HOC轮廓数密度为(56.20±40.40),肝纤维化组肝小叶内HOC轮廓数密度为(566.50±317.00),与对照组比较,肝纤维化组大鼠肝小叶内轮廓数密度显著增加了9.08倍(t=-3.539,P=0.006);定性观察研究结果显示,肝纤维化大鼠肝纤维化分期按照Metavir评分标准达到Ⅱ~Ⅲ期,大鼠窦周隙内肝星状细胞增生部位周围伴随着HOC的大量增生。  结论  CCl4诱导肝纤维化大鼠肝小叶内HOC显著增生。

     

  • 图  1  体视框计数肝卵圆细胞数密度

    注: 黑色箭头示肝HOC;红色箭头示肝细胞。体视框面积为2 354.45 μm2,红线为禁线,绿线为计数线,计数落在体视框内、或与绿线相交的肝HOC,落在红线上的肝HOC不计数。

    Figure  1.  Contour number density of hepatic oval cells counted by stereoscopic frame

    图  2  Masson染色的肝组织结构

    注: ①胶原纤维;②中央静脉;③汇管区;④肝细胞;⑤肝血窦。

    Figure  2.  Histological structure of liver by Masson staining

    图  3  电镜下肝组织超微结构

    注: ①HOC;②肝细胞;③肝血窦;④肝星状细胞;⑤窦周隙。

    Figure  3.  Ultrastructure of liver tissue under electron microscope

    图  4  电镜下HOC超微结构(×7 000)

    注: ①肝卵圆细胞;②胶原纤维;③线粒体;④粗面内质网。

    Figure  4.  Ultrastructure of hepatic oval cell under electron microscope(×7 000)

    表  1  各组大鼠肝小叶内肝HOC总体积与轮廓数密度(每mm2细胞数量)变化

    Table  1.   Changes in total volume (mm3) and contour number density (number of cells per mm2) of hepatic oval cells in hepatic lobules of rats in each group

    项目 对照组(n=5) 肝纤维化组(n=6) t P
    肝卵圆细胞轮廓数密度 56.20±40.40 566.50±317.00 -3.539 0.006
    肝卵圆细胞总体积(mm3 15.40±7.63 146.80±114.00 -2.551 0.031
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  • 收稿日期:  2023-03-22
  • 录用日期:  2023-06-05
  • 出版日期:  2024-01-23
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