慢性HBV感染者肝脏HBV cccDNA含量相关因素分析

陈嵬; 吴峰; 窦晓光; 张琳

慢性HBV感染者肝脏HBV cccDNA含量相关因素分析

1. 中国医科大学附属盛京医院感染科
2. 大连市第六人民医院

基金项目:

国家“十一五”传染病重大专项课题(2008ZX10002-004)；国家自然科学基金项目(30972612)；

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中图分类号: R512.62
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出版历程
- 出版日期: 2013-06-20

Analysis of related factors for intrahepatic HBV cccDNA level in patients with chronic HBV infection

Department of Infectious Diseases, Shengjing Hospital of China Medical University

Research funding:

摘要

摘要:
目的探讨慢性HBV感染者肝组织中HBV cccDNA含量与血清病毒标志物、HBV DNA及肝脏病理分级的关系,为临床评价抗病毒治疗效果及疗程确定提供理论依据。方法以2007年5月-2008年2月住院的30例慢性HBV感染者为研究对象,应用实时荧光定量聚合酶链反应（RT-PCR）方法检测患者肝组织中HBV cccDNA、肝组织总HBV DNA（HBV tDNA）和血清HBVDNA,同时用化学发光免疫分析法检测HBsAg、HBeAg定量,分析感染者肝组织内HBV cccDNA与肝组织内HBV DNA、血清HBVDNA、HBsAg及HBeAg定量水平之间的关系,并比较肝组织中HBV cccDNA含量与肝脏病理炎症和纤维化分级的关系。采用Pear-son简单相关和Spearman等级相关法进行相关性分析。结果 30例慢性HBV感染者肝组织中均可检出HBV cccDNA,范围在3.15×103～1.06×107拷贝/mg;肝组织cccDNA定量与肝组织总HBV DNA定量呈正相关（r=0.375,P<0.05）,与血清hbv r="0.174,P">0.05）;肝组织中H...
- 肝炎病毒,乙型 /
- DNA,环状 /
- 病理过程
Abstract:
Objective To investigate the correlations of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) level in liver tissue with serum viral markers, HBV DNA levels, and pathological changes of liver tissue in patients with chronic HBV infection, and to provide a theoretical basis for evaluating the efficacy and determining the course of antiviral therapy.Methods Thirty patients with chronic HBV infection who were hospitalized from May 2007 to February 2008 were included in the study.The HBV cccDNA in liver tissue, total HBV DNA (HBV tDNA) in liver tissue, and serum HBV DNA were quantified by real-time quantitative polymerase chain reaction;the HBsAg and HBeAg in serum were quantified by chemiluminescence immunoassay.The correlations of HBV cccDNA level in liver tissue with HBV tDNA level in liver tissue, serum HBV DNA level, serum HBsAg and HBeAg levels, and the inflammation activity and fibrosis degree of liver tissue were analyzed;Pearson's simple correlation coefficient and Spearman's rank correlation coefficient were used for correlation analysis.Results HBV cccDNA was detected in all the 30 patients with chronic HBV infection, with a content ranging from 3.15×103 to 1.06×107 copies/mg.The HBV cccDNA level in liver tissue had a positive correlation with HBV tDNA level in liver tissue (r=0.375, P<0.05) but="" had="" no="" correlation="" with="" serum="" hbv="" dna="" level="" r="0.174," p="">0.05) .The HBV cccDNA level in liver tissue had a highly positive correlation with serum HBsAg level (r=0.562, P<0.001) but="" had="" no="" correlation="" with="" serum="" hbeag="" level="" r="0.152," p="">0.05) .The HBV cccDNA level in liver tissue was not correlated with the inflammation activity and fibrosis degree of liver tissue (r=0.082, P>0.05) .Conclusion The replication of HBV cccDNA is stable and moderate in patients with chronic HBV infection.Serum HBV DNA load cannot directly represent the HBV cccDNA level in liver tissue;serum HBsAg level can be used as an indicator of HBV cccDNA level in liver tissue.
- hepatitis B virus /
- pathology of liver tissue DNA /
- civcular

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参考文献(13)

[1]Locarnini S, Zoulim F.Molecular genetics of HBV infection[J].Antivir Ther, 2010, 15 (13) :3-14.

[2]Moucari R, Marcellin P.HBsAg seroclearance:prognostic value forthe response to treatment and the long-term outcome[J].Gastroen-terol Clin Biol, 2010, 34 (2) :S119-125.

[3]Chinese Society of Hepatology and Chinese Society of Infecions Dis-ease, Chinese Medilal Association.The guideline of prevention andtreatment for chronic hepatitis B[J].J Clin Hepatol, 2006, 22 (1) :3-15. (in Chinese) 中华医学会肝病学分会, 中华医学会感染病学会.慢性乙型肝炎防治指南[J].临床肝胆病杂志, 2006, 22 (1) :3-15.

[4]Quasdorff M, Protzer U.Control of hepatitis B virus at the level of tran-scription[J].J Viral Hepat, 2010, 17 (8) :527-536.

[5]Danri M, Burda MR, Will H, et al.Increased hepatocyte turnoverand inhibition of woodchuck hepatitis B virus replication by adefovirin vitro do not lead to reduction of the closed circular DNA[J].Hep-atology, 2000, 32 (1) :139-146.

[6]Delmas J, Schorr O, Jamard C, et al.Inhibitory effect of adefovir onviral DNA synthesis and covalently closed circular DNA formation induck hepatitis B virus-infected hepatocytes in vivo and in vitro[J].Antimicrob Agents Chemother, 2002, 46 (2) :425-433.

[7]Werle-Lapostolle B, Bowden S, Locarnini S, et al.Persistence ofcccDNA during the natural history of chronic hepatitis B and declineduring adefovir dipivoxil therapy[J].Gastroenterology, 2004, 126 (7) :1750-1758.

[8]Schultz U, Chisari FV.Recombinant duck interferon gamma inhib-its duck hepatitis B virus replication in primary hepatocytes[J].JVirol, 1999, 73 (4) :3162-3168.

[9]Liu C, Mu YP, Yang ZG, et al.Clinical significance of monitoringhepatitis B virus Core-related antigens[J].J Clin Hepatol, 2012, 28 (10) :793-796. (in Chinese) 刘成, 慕永平, 杨宗国, 等.乙型肝炎病毒核心相关抗原的监测及临床意义[J].临床肝胆病杂志, 2012, 28 (10) :793-796.

[10]Yuen MF, Wong DK, Sablon E, et al.HBsAg seroclearance in chronichepatitis B in the Chinese:virological, histological, and clinical as-pects[J].Hepatology, 2004, 39 (6) :1694-1701.

[11]Zoulim F.New insight on hepatitis B virus persistence from thestudy of intrahepatic viral cccDNA[J].J Hepatol, 2005, 42 (3) :302-308.

[12]Yuen MF, Wong DK, Fung J, et al.HBsAg seroclearance in chro-nic hepatitis B in Asian patients:replicative level and risk of hepa-tocellular carcinoma[J].Gastroenteroly, 2008, 135 (4) :1192-1199.

[13]Tuttleman JS, Pourcel C, Summers J.Formation of the pool of co-valently closed circular viral DNA in hepadnavirus-infected cells[J].Cell, 1986, 47 (3) :451-460.

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