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脂联素对大鼠原代Kupffer细胞白细胞介素10表达的影响

李欣 陈辉 李红艺 王瑞丽 孙淑杰 任江波 马红

引用本文:
Citation:

脂联素对大鼠原代Kupffer细胞白细胞介素10表达的影响

基金项目: 

中国肝炎防治基金会王宝恩肝纤维化研究基金资助项目(20070027); 

详细信息
  • 中图分类号: R575

Effects of adiponectin on IL-10 expression in rat primary Kupffer cells

Research funding: 

 

  • 摘要:

    目的本研究观察外源性脂联素对体外培养的大鼠原代Kupffer细胞白细胞介素(IL)-10表达的调节作用,以阐明脂联素的抗炎作用机制。方法通过两步胶原酶灌流、密度梯度离心和2 h选择性贴壁方法分离纯化大鼠肝脏Kupffer细胞。酶联免疫吸附试验方法检测脂联素作用下原代Kupffer细胞上清液中IL-10的表达水平。荧光定量PCR法检测脂联素作用下原代Kupffer细胞IL-10 mRNA表达。结果 0.5、1.0、1.5μg/ml脂联素处理Kupffer细胞4、8、12、16 h,与对照组相比,同一浓度的脂联素随着培养时间的延长,Kupffer细胞IL-10分泌逐渐增加;而同一作用时间下,随着脂联素浓度的增加,Kupffer细胞IL-10分泌量并没有逐渐增加。1.0和1.5μg/ml脂联素作用Kupffer细胞16 h,IL-10基因表达量与对照组相比显著增加,各处理组间无明显差异。结论脂联素可上调大鼠原代Kupffer细胞IL-10的表达,这可能是其抗炎作用机制之一。

     

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  • 出版日期:  2013-04-20
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