超声弹性成像评价大鼠脂肪肝分级的应用价值

唐少珊; 赵国家; 王一娇

超声弹性成像评价大鼠脂肪肝分级的应用价值

中国医科大学附属盛京医院超声科

基金项目:

沈阳市科学技术计划项目(F10-205-1-07)；

详细信息

中图分类号: R575.5
计量
- 文章访问数: 3000
- HTML全文浏览量: 9
- PDF下载量: 607
- 被引次数: 0
出版历程
- 出版日期: 2013-04-20

Assessment of ultrasound elastography to grade hepatic steatosis in a rat model

Research funding:

摘要

摘要:
目的探讨实时超声弹性成像评价大鼠脂肪肝分级的应用价值。方法建立大鼠脂肪肝模型,分别于造模第6、8、10、12、14周随机选取大鼠进行超声弹性测量。在超声弹性成像模式下获取参照物与大鼠肝脏的应变比(B/A),然后处死大鼠,取出肝脏,进行病理切片并进行脂肪肝的分级诊断。最后将所获取的大鼠肝脏弹性数据与病理显示的脂肪浸润程度分级进行相关性分析。结果实验最终获得40只大鼠的超声弹性数据和病理结果,不同脂肪肝病理分级的B/A两两比较差异均有统计学意义(P均<0.05);B/A与其病理分级间呈相关性(r=0.88,P<0.01)。结论实时超声弹性成像为无创定量诊断脂肪肝提供了新方法,将在临床中有良好的应用前景。
- 弹性成像技术 /
- 脂肪肝 /
- 模型,动物
Abstract:
Objective To explore whether the non-invasive method of ultrasound elastography can diagnose and grade hepatic steatosis by using a rat model system.Methods Fifty healthy male Wistar rats were divided into a model group (n=40) and a control group (n=10) .The model was induced by high-fat diet (20% lard) , alcohol intake (10% in drinking water) , and CCl4 subcutaneous injections (40% in olive oil;0.2 ml/kg two times a week) .The control rats received normal diet, no alcohol, and olive oil injections.After weeks 6, 8, 10, 12, and 14 of the regimen, rats from each group were randomly selected and subjected to liver ultrasound elastography analysis (Hitachi HV900 Color Doppler instrument;6-13 MHz linear probe frequency) , first as transabdominal imaging then by fully exposed rat liver imaging, followed by pathological examination with hematoxylin-eosin staining.The imaging techniques were performed in triplicate for each rat and averaged.The imaging findings of controls (B) were compared to those of models (A) and used to calculate the strain ratio (B/A) .Hepatic steatosis detected by pathology was graded as percent of fatty degeneration: normal,<5%;mild,>66%.The correlations between B/A and pathology grades were assessed by Kruskal-Wallis and Spearman's coefficient tests.Results Ten rats died during the establishment of the hepatic steatosis model system.Of the 30 surviving model rats assessed by ultrasound and pathology, the hepatic steatosis grade was mild in 10, moderate in 12, and severe in 8;all control rats showed normal liver.The transabdominal imaging results (B/A) were significantly different between control rats (0.25±0.04) and rats with all three grades of hepatic steatosis (vs.mild: 0.43±0.05, moderate: 0.59±0.17, and severe: 0.89±0.21;P<0.05) .The B/A was positively correlated with the pathology grade (r=0.88, P<0.001) .Conclusion Ultrasonic elastography effectively diagnosed hepatic steatosis and was able to quantitate various pathologic grades in a non-invasive manner, suggesting its potential utility for clinical application.
- elasticity imaging techniques /
- fatty liver /
- model

HTML全文

参考文献(16)

[1]Zou ZQ, Qiang CQ, Li YF, et al.The clinical features and re-lated factors of chronic hepatitis B with hepoctic steatosis[J].J Clin Hepatol, 2011, 27 (6) :620-622. (in Chinese) 邹志强, 强春倩, 李艳芳, 等.慢性乙型肝炎合并肝脂肪变的临床病理学特点及相关因素分析[J].临床肝胆病杂志, 2011, 27 (6) :620-622.

[2]Streba LA, C arstea D, Mitru爫P, et al.Nonalcoholic fatty liv-er disease and metabolic syndrome:A concise review[J].Rom J Morphol Embryol, 2008, 49 (1) :13-20.

[3]Farrell, Larter.Nonalcoholic fatty liver disease:From steato-sis to cirrhosis[J].Hepatology, 2006, 43 (2 Suppl 1) :S99-S112.

[4]Brunt EM.Pathology of nonalcoholic fatty liver disease[J].Nat Rev Gastroenterol Hepatol, 2010, 7 (4) :195-203.

[5]Mehta SR, Thomas EL, Bell JD, et al.Non-invasive meansof measuring hepatic fat content[J].World J Gastroenterol, 2008, 14 (22) :3476-3483.

[6]Fu LN, Wang Y, Wang Y, et al.Value of ultrasound elastog-raphy in detecting small breast tumors[J].Chin Med J (En-gl) , 2011, 124 (15) :2384-2386.

[7]Fan JG, Qiao L.Commonly used animal models of non-al-coholic steatohepatitis[J].Hepatobiliary Pancreat Dis Int, 2009, 8 (3) :233-240.

[8]Merat S, Khadem SF, Nouraie M, et al.A modification ofthe Brunt system for scoring liver histology of patients withnon-alcoholic fatty liver disease[J].Arch Iran Med, 2010, 13 (1) :38-44.

[9]de Moura Almeida A, Cotrim HP, Barbosa DB, et al.Fattyliver disease in severe obese patients:diagnostic value of ab-dominal ultrasound[J].World J Gastroenterol, 2008, 14 (9) :1415-1418.

[10]Tobari M, Hashimoto E, Yatsuji S, et al.Imaging of nonalco-holic steatohepatitis:Advantages and pitfalls of ultrasonogra-phy and computed tomography[J].Intern Med, 2009, 48 (10) :739-746.

[11]Borra RJ, Salo S, Dean K, et al.Nonalcoholic fatty liver dis-ease:Rapid evaluation of liver fat content with in-phase andout-of-phase MR imaging[J].Radiology, 2009, 250 (1) :130-136.

[12]Mai J, Gao YQ.The application of magnetic resonace elastogra-phy in the non-invasive diagnosis of hepatic fibrosis[J].J ClinHepatol, 2011, 27 (3) :318-320, 324. (in Chinese) 麦静, 高脙.磁共振弹性成像无创检查在肝纤维化诊断中的应用[J].临床肝胆病杂志, 2011, 27 (3) :318-320, 324.

[13] Ophir J, Céspedes I, Ponnekanti H, et al.Elastography:Aquantitative method for imaging the elasticity of biological tis-sues[J].Ultrason Imaging, 1991, 13 (2) :111-134.

[14]Gheonea DI, Sftoiu A, Ciurea T, et al.Real-time sono-elastography in the diagnosis of diffuse liver diseases[J].World J Gastroenterol, 2010, 16 (14) :1720-1726.

[15]Li YY, Wang XM, Zhang YX, et al.Ultrasonic elastographyin clinical quantitative assessment of fatty liver[J].World JGastroenterol, 2010, 16 (37) :4733-4737.

[16]Gheorghe L, Iacob S, Gheorghe C.Real-time sonoelastog-raphy—a new application in the field of liver disease[J].JGastrointestin Liver Dis, 2008, 17 (4) :469-474.

施引文献

资源附件(0)

访问统计