干扰素治疗慢性乙型肝炎患者HBeAg转阴率和CD4+CD25+调节性T淋巴细胞水平的关系

蒋淑莲; 谭善忠; 文剑

干扰素治疗慢性乙型肝炎患者HBeAg转阴率和CD4+CD25+调节性T淋巴细胞水平的关系

东南大学附属第二医院

基金项目:

国家自然科学基金(81000997)；第50批中国博士后基金(2011M500843)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2012-07-20

Role of CD4+CD25+ T regulatory cells in HBeAg seroconversion during interferon antiviral

Research funding:

摘要

摘要: 目的观察干扰素抗病毒治疗前后慢性乙型肝炎患者外周血CD4+CD25+调节性T细胞（CD4+CD25+Treg）表达水平的变化和HBeAg转阴的关系。方法选择用干扰素α-2b治疗的HBeAg阳性慢性乙型肝炎56例,分别于治疗前、48周时检测外周静脉血CD4+CD25+Treg占CD4+T细胞的比例,同时检测HBeAg、ALT和HBV DNA载量。结果抗病毒治疗48周后HBeAg转阴组CD4+CD25+Treg水平[（9.12±2.45）%]较治疗前[（11.99±3.25）%]明显下降（P<0.01）;而HBeAg未转阴组较治疗前无明显下降（P>0.05）。结论慢性乙型肝炎患者经干扰素α-2b抗病毒治疗,HBeAg血清转换者CD4+CD25+Treg水平降低,而未转阴者Treg下降不明显。
- 肝炎e抗原,乙型 /
- 干扰素 /
- T淋巴细胞,调节性
Abstract: Objective To investigate the role of CD4+CD25+ T regulatory cells in hepatitis B e antigen (HBeAg) serological conversion during anti-hepatitis B virus (HBV) treatment using interferon.Methods Fifty-six HBeAg-positive chronic HBV patients were administered interferon-alpha 2b (5 000 000 U) intramuscularly every other day for one year.CD4+CD25+ T regulatory cells were detected by flow cytometry before treatment and 48 weeks after treatment initiation.Results The level of CD4+CD25+ T regulatory cells was significantly reduced by interferon-alpha 2b treatment in patients with HBeAg conversion (before: 11.99%±3.25% vs 48 weeks: 9.12%±2.45%;P<0.05) .However, no decrease in the level of T regulatory cells was observed in the group without HBeAg conversion.Conclusion In chronic HBV, interferon treatment decreases the level of CD4+CD25+ T regulatory cells in patients with HBeAg seroconversion, but not in patients without HBeAg seroconversion.
- hepatitis B e antigens /
- interferon /
- T-lymphocytes

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参考文献(8)

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