Abstract: Objective To explore the clinical feasibility of using a posterior Calot′s triangle approach for three-port laparoscopic cholecystectomy (LC) .Methods The clinical data (operative time, blood loss, and complications) of 25 patients who underwent three-port LC with the posterior Calot′s triangle approach were analyzed retrospectively.Results All operations had been performed successfully, and none required conversion to the four-trocar method.The operative time ranged from 25 min to 60 min (mean: 40 min) .The blood loss volume ranged from approximately 1 ml to 10 ml (mean: 3 ml) .There were no reports of severe complications, including bile duct injury, bile leakage, or hemorrhage, for any of the 25 cases.During follow-up (range: 413 months;mean: 7 months) , no bile duct stenosis or biliary tract symptoms were reported.Conclusion The posterior Calot′s triangle approach for LC effectively allowed for the identification and separation of anatomical structures in Calot′s triangle, while avoiding injury to the vessels and bile duct.The three-port LC with the posterior Calot′s triangle approach is safe and feasible.