Abstract: Objective To investigate the diagnostic value of blood platelet (PLT) and hyaluronic acid (HA) levels for cirrhosis.Methods Coded peripheral blood samples obtained from chronic hepatitis B virus (HBV) infection patients (n=233) and HBV-related liver cirrhosis patients (n=144) were used to measure blood PLT counts by an automated blood cell analyzer and serum HA levels by chemiluminescent assay.Correlation of PLT counts and HA levels with HBV-related cirrhosis was analyzed by receiver operating characteristic (ROC) curve analysis.Results For PLT, the area under the curve (AUC) was 0.888±0.0165.The PLT cutoff value was determined to be≤91×109/L, which provided diagnostic sensitivity of 84.03% and specificity of 82.83%.Meanwhile, the HA AUC was 0.920±0.0138.The HA cutoff value was determined to be>308 nmol·ml-1·h-1, which provided diagnostic sensitivity of 84.72% and specificity of 88.84%.The AUCs of PLT and HA were not significantly different (P=0.089) , suggesting that both PLT and HA are excellent indexes of cirrhosis.Furthermore, the AUC of log10 (PLT/HA) was 0.945±0.0113 and the cutoff value was determined to be ≤-0.595, which provided diagnostic sensitivity of 88.19% and specificity of 89.7%.The AUC of log10 (PLT/HA) and PLT (P=0.0008) was significantly different from the AUC of log10 (PLT/HA) and HA (P<0.0001) , suggesting that combined detection of PLT and HA will be more valuable for diagnosis of cirrhosis.Conclusion Combined detection of PLT and HA represents a reliable method to diagnose cirrhosis in patients with hepatitis B, and may be especially useful in cases lacking histopathologic findings.