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Smad3 shRNA抑制TGFβ1对HSC-T6细胞活化的抗肝纤维化作用

郑素军 邢欣悦 武聚山 王世美 张莹 韩源平 俞豪 陈煜 刘梅 段钟平

郑素军, 邢欣悦, 武聚山, 王世美, 张莹, 韩源平, 俞豪, 陈煜, 刘梅, 段钟平. Smad3 shRNA抑制TGFβ1对HSC-T6细胞活化的抗肝纤维化作用[J]. 临床肝胆病杂志, 2012, 28(4): 289-295.
引用本文: 郑素军, 邢欣悦, 武聚山, 王世美, 张莹, 韩源平, 俞豪, 陈煜, 刘梅, 段钟平. Smad3 shRNA抑制TGFβ1对HSC-T6细胞活化的抗肝纤维化作用[J]. 临床肝胆病杂志, 2012, 28(4): 289-295.
Zheng SuJun, Xing XinYue, Wu JuShan, Wang ShiMei, Zhang Ying, Han YuanPing, Yu Hao, Chen Yu, Liu Mei, Duan ZhongPing. ShRNA targeted to rat Smad3 decreases the profibrosis role of TGFβ1 in HSC-T6 cells[J]. J Clin Hepatol, 2012, 28(4): 289-295.
Citation: Zheng SuJun, Xing XinYue, Wu JuShan, Wang ShiMei, Zhang Ying, Han YuanPing, Yu Hao, Chen Yu, Liu Mei, Duan ZhongPing. ShRNA targeted to rat Smad3 decreases the profibrosis role of TGFβ1 in HSC-T6 cells[J]. J Clin Hepatol, 2012, 28(4): 289-295.

Smad3 shRNA抑制TGFβ1对HSC-T6细胞活化的抗肝纤维化作用

基金项目: 

国家自然科学基金资助项目(30800979); 北京自然科学基金资助项目(7102085); 北京市科技新星资助项目(2007B055); 北京市卫生系统高层次卫生人才培养计划(2011-3-083); 北京市教育委员会科技发展计划项目(KM201010025024); 

详细信息
  • 中图分类号: R575.2

ShRNA targeted to rat Smad3 decreases the profibrosis role of TGFβ1 in HSC-T6 cells

Research funding: 

 

  • 摘要: 目的观察Smad3 shRNA对TGFβ1作用于大鼠HSC-T6细胞增殖、细胞周期以及分泌肝纤维化胶原的影响。方法Smad3 shRNA慢病毒感染HSC-T6,应用流式细胞仪检测其感染率,Real-time PCR检测对Smad3 mRNA表达的抑制作用;将HSC-T6对照、HSC-T6+TGFβ1(10 ng/ml)、Smad3 shRNA以及Smad3 shRNA+TGFβ1(10 ng/ml)这4组细胞进行如下检测:(1)MTT检测细胞增殖;(2)流式细胞仪检测TGFβ1作用24、36 h细胞周期分布;(3)Real-time PCR检测24、36 h时细胞周期、增殖及肝纤维化相关基因mRNA的表达变化;(4)ELISA检测24、36 h培养液上清中胶原蛋白(COL)Ⅰ、COLⅢ及α-SMA的含量。结果Smad3 shRNA慢病毒感染HSC-T6细胞至96 h,感染率为71.3%,对Smad3 mRNA的表达抑制率为50%。(1)MTT结果显示,TGFβ1促进HSC-T6增殖;与相应未感染Smad3 shRNA病毒组相比,Smad3 shRNA组以及Smad3 shRNA+TGF...

     

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  • 出版日期:  2012-04-20
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