The study on relationship of basic fibroblast growth factor with the clinical significance on chronic viral hepatitis B and liver fibrosis
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摘要: 目的观察碱性成纤维细胞生长因子(b-FGF)在不同肝纤维化分期(S)肝组织中表达的差异性及其与临床检测常用的肝功能指标、肝纤维化指标、HBV DNA复制水平以及肝硬化Child-Pugh分级之间的关系。方法选取122例HBV引起的慢性肝炎及肝硬化患者,空腹静脉血检测:(1)肝功能指标:ALT、TBil、Alb;(2)肝纤维化指标:透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、层粘连蛋白(LN)、Ⅳ型胶原(CⅣ);(3)HBV DNA复制水平;(4)对临床诊断肝硬化患者计算其Child-Pugh评分。行肝脏穿刺活检术后,经病理作纤维化分期(S1~S4)。免疫组织化学法测定肝组织中b-FGF在不同病理分期的表达及定位特点,半定量分析法计算b-FGF值,观察b-FGF与肝功能指标、肝纤维化指标、HBV DNA水平以及肝硬化Child-Pugh分级之间的关系。结果 (1)肝纤维化S1~S4期肝组织中b-FGF的表达部位及水平不同;(2)肝纤维化S1~S4期肝细胞中b-FGF的表达与肝纤维化分期(S1~S4)呈显著正相关(r=0.542,P<0.01);(3)不同肝纤维化分期中b-FGF的表达差...
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关键词:
- 成纤维细胞生长因子2 /
- 肝硬化 /
- 肝炎,乙型,慢性
Abstract: Objective To observe expressions of b-FGF in different stage on the hepatic fibrosis and to discuss the relationship with indexes of hepatic function, indexes of hepatic fibrosis, hepatic cirrhosis Child-Pugh classification, HBV DNA replication.Methods 122 cases of HBV-induced chronic hepatitis and cirrhosis were selected.Delect hepatic function (ALT, TBil, Alb) , indexes of liver fibrosis: hyaluronic acid (HA) , Ⅲ procollagen (PC Ⅲ) , laminin (LN) , Ⅳ collagen (CⅣ) , HBV DNA replication were tested and hepatic cirrhosis Child-Pugh classification were judged.the pathological diagnosis and different stages of the hepatic fibrosis of 122 patients with chronic hepatitis were determined by liver biopsy.b-FGF Value was calculated, the relationship of b-FGF with indexes of hepatic function, indexes of hepatic fibrosis, HBV DNA replication, hepatic cirrhosis Child-Pugh classification was observed.Results 1.There were some difference of expressions of b-FGF in different parts and levels of liver fibrosis S1-S4 stage.2.The expressions of b-FGF were gradually increased with the development of the hepatic fibrosis (S1-S4) and positive correlation was determined (r=0.542, P<0.01) .3.In the different processes of the hepatic fibrosis (S1-S4) the expressions of b-FGF with ALT, TBil, ALB, HBV DNA replication were not statistically significant (P>0.05) ;4.In the different processes of the hepatic fibrosis (S1-S4) the expressions of b-FGF with HA, PC Ⅲ, LN, CⅣ were positively correlated.5.The expressions of b-FGF and Child-Pugh classification had no correlation.Conclusion The expressions of b-FGF have a positive correlation with the level of hepatic fibrosis and indexes of hepatic fibrosis and there is no correlation of expressions of b-FGF with ALT, TBil, Alb, Child-Pugh score and HBV-DNA replication.-
Key words:
- fibroblast growth factor 2 /
- liver cirrhosis /
- hepatitis B
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