组蛋白去乙酰化酶1及基质金属蛋白酶-2在肝细胞癌组织中的表达及意义

王迎春; 刘炎芳; 朱瑞萍; 宋欣新; 李小欢

组蛋白去乙酰化酶1及基质金属蛋白酶-2在肝细胞癌组织中的表达及意义

1. 大连大学附属中山医院消化内科
2. 大连大学附属中山医院病理科
3. 大连大学附属中山医院中心实验室

基金项目:

大连市医学卫生科学研究计划项目(2011178)；

详细信息

中图分类号: R735.7
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出版历程
- 出版日期: 2012-04-20

Expression of histone deacetylase 1 and matrix metalloproteinase-2 in hepatocellular carcinoma and its significance

Research funding:

摘要

摘要: 目的探讨组蛋白去乙酰化酶1（HDAC1）及基质金属蛋白酶-2（MMP-2）在肝细胞癌（HCC）患者肝组织中的表达及其临床意义。方法采用免疫组织化学方法检测42例HCC和相应的癌旁组织中HDAC1及MMP-2蛋白的表达,并分析其与临床病理参数的关系。结果 HCC组织中,HDAC1及MMP-2蛋白的表达明显高于癌旁组织（P<0.01,P<0.05）;HDAC1蛋白的表达与患者性别、年龄、肿瘤大小、AFP浓度、肿瘤分化程度、脉管癌栓无关联（P>0.05）,与肿瘤TNM分期相关联（P<0.05）;MMP-2蛋白的表达与患者性别、年龄、肿瘤大小、AFP浓度、肿瘤分化程度无关联（P>0.05）,与脉管癌栓、肿瘤TNM分期相关联（P<0.05,P<0.01）;HCC组织中,HDAC1及MMP-2蛋白的表达呈正相关（P<0.01）。结论 HDAC1及MMP-2在HCC患者肝组织中表达有一定的肿瘤特异性,二者的高表达提示HCC已属晚期。
- 癌,肝细胞 /
- 组蛋白去乙酰化酶1 /
- 基质金属蛋白酶类
Abstract: Objective To study the expression of HDAC1 and MMP-2 in liver tissues of patients with hepatocellular carcinoma (HCC) and its significance.Methods Immunohistochemical analysis was used to detect the expression of HDAC1 and MMP-2 in 42 cases of HCC.Results The positive rate of HDAC1 and MMP-2 was significantly higher in HCC than that in para-cancerous tissues (P<0.01, P<0.05) , the expression of HDAC1 was not associated with patients′ gender, age, tumor size, alpha fetoprotein, histological grade and vessel embolus (P>0.05) , but was significantly associated with patients′ TNM stage (P<0.05) , the expression of MMP-2 was not associated with patient gender, age, tumor size, alpha fetoprotein, histological grade (P>0.05) , but was significantly associated with patient vessel embolus and TNM staging (P<0.05, P<0.01) , HDAC1 was positively correlated with MMP-2 expression in HCC.Conclusion In HCC tissues, increased HDAC1 and MMP-2 expression may associate to the hepatocarcinogenesis, and may indicate the status of advanced TNM stage.
- carcinoma /
- hepatocellular /
- histonedeacetylases1

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参考文献(10)

[1]Gallinari P, Dimarco S, Jones P, et al.HDACs, histone deacetyla-tion and gene transcription:from molecular biology to cancer ther-apeutics[J].Cell Res, 2007, 17 (3) :195-211.

[2] Poon D, Anderson BO, Chen LT, et al. Management of hepatocellular carcinoma in Asia: consensus statement from the Asian Oncology Summit 2009[J]. Lancet Oncol, 2009, 10 (11) : 1111-1118.

[3] Mariadason JM. HDACs and HDAC inhibitors in colon cancer[J]. Epigenetics, 2008, 3 (1) : 1-10.

[4] Bertrand P. Inside HDAC with HDAC inhibitors[J].Eur J Medchem, 2010, 45 (6) : 2095-2116.

[5] Taunton J. Hassig CA, Schreiber SL. A mammalion histone deacetylase related to the yeast transcriptional regulator Rpd3p[J]. Science, 1996, 272 (5) : 408-411.

[6]Rikimaru T, Taketomi A, Yamashita Y, et al.Clinical significance of histone deacetylase1expression in patients with hepatocel-lular carcinoma[J].Oncology, 2007, 72 (1-2) :69-74.

[7]Yoo YG, Na TY, Seo HW, et al.Hepatitis B virus X protein induces the expression of MTA1and HDAC1, which en-hances hypoxia signaling in hepatocellular carcinoma cells[J].Oncogene, 2008, 27 (24) :3405-3413.

[8] Zopf S, Neureiter D, Bouralexis S, et al. Differential response of p53 and p21 on HDAC inhibitor mediated apoptosis in HCT116 colon cancer cells in vitro and in vivo [J]. Int Oncol, 2007, 31 (16) :1391-1402.

[9] Giannelli G, Bergamini C, Marinosci F, et al. Clinical role of MMP-2/ TIMP-2 imbalance in hepatocellular carcinoma[J]. Int J Cancer, 2002, 97 (4) :425-431.

[10]whetstine JR, Ceron J, Ladd B, et al.Regulation of tissue specific and extracellular matrix-related genes by a class1 histone deacetylase[J].Mol Cell, 2005, 18 (4) :483-490.

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