前列腺素E1抑制慢性乙型重型肝炎树突状细胞成熟及CD4+/CD8+T细胞活性的观察

石庆凤; 张颖新; 宋吉奎; 袁建国; 李庆方; 赵红奎; 曾维群; 陈敏

前列腺素E1抑制慢性乙型重型肝炎树突状细胞成熟及CD4+/CD8+T细胞活性的观察

1. 聊城市人民医院感染科
2. 聊城市人民医院中心实验室
3. 重庆医科大学第二附属医院

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中图分类号: R512.62
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- 出版日期: 2012-03-20

Effect of prostaglandin E1 on the dendritic cells maturation and the activity of CD4+/CD8+ T cells in chronic severe hepatitis B patients

摘要

摘要: 目的研究前列腺素E1对慢性乙型重型肝炎树突状细胞成熟及CD4+/CD8+T细胞活性的影响。方法抽取前列腺素E1治疗10 d前后的慢性乙型重型肝炎患者外周静脉血,密度梯度离心法分离淋巴细胞,贴壁培养获得PBMC,经重组人白细胞介素4（rhIL-4）、重组人粒-巨噬细胞集落刺激因子（rhGM-CSF）、加或不加前列腺素E1刺激培养8 d获得树突细胞（DC）。经流式细胞仪检测DC表达的HLA-DR、CD83、CD86,ELISA测定分泌的IFNγ、IL-12p70。悬浮T细胞部分检测CD4+/CD8+T细胞比例及其细胞表面CD69、HLA-DR表达;部分经IL-2培养,用于检测DC体外诱导的T淋巴细胞毒活性。结果前列腺素E1治疗慢性乙型重型肝炎,对患者CD4+/CD8+T细胞比例无明显影响,可抑制CD4+/CD8+T细胞活化分子CD69、HLA-DR的表达;前列腺素E1体外培养的DC低表达HLA-DR、CD83、CD86,分泌IFNγ、IL-12p70均下降,体外诱导的自体淋巴细胞毒活性减弱,与未加用前列腺素E1比较差异有统计学意义（P<0.01）。结论前列腺素E1治疗慢性乙型重型肝炎...
- 前列地尔 /
- 肝炎,乙型,慢性 /
- 树突细胞
Abstract: Objective To study the maturation of dendritic cells and the activity of CD4+/CD8+T cells after prostaglandin E1 treatment in chronic severe hepatitis B patients.Methods PBMC were isolated from fresh peripheral blood of chronic severe hepatitis B patients by Ficoll-Hypaque density gradient centrifugation and cultured with plastic-adherence method.Granulocyte-macrophage colony stimulating factor and interleukin-4 with or without prostaglandin E1 were used to induce the PBMC to obtain dendritic cells for 8 days.FACS was used to analyze the expression of the DC surface markers, including human leukocyte antigen (HLA-DR) , CD83, CD86.The secretion of IFN-γ, IL-12p70 was detected by ELISA.Some of the non-adherent cells were used to analyze the ratio of CD4+/CD8+T cells and the expression of CD69 and HLA-DR, the others were incubated with IL-2 for analyzing the cytotoxic T lymphocyte activity.Results Although prostaglandin E1 had no effects on the activity of CD4+/CD8+T cells in chronic severe hepatitis B patients, it could decrease the expression of CD4+/CD8+T cells activators, such as CD69 and HLA-DR.Further more, compared with the non-prostaglandin E1 control group, prostaglandin E1could down-regulate the expression of the surface markers on dendritic cells, including HLA-DR, CD83 and CD86, and inhibit the secretion of IL-12p70.In addition, prostaglandin E1 could inhibit the cytotoxic activity of the T lymphocyte in vitro.Conclusion Prostaglandin E1 inhibited the activation of CD4+/CD8+T cells, the maturation of dendritic cells and the cytotoxic activity of T lymphocytes.
- alprostadil /
- hepatitis B /
- chronic

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参考文献(12)

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