HBeAg（+）与HBeAg（-）乙型肝炎病毒携带者外周血CD4+CD25+调节性T淋巴细胞比例的变化及意义

吴银亚; 谭善忠; 赵磊; 孙薇薇; 沈建军; 梁重锋

HBeAg（+）与HBeAg（-）乙型肝炎病毒携带者外周血CD4+CD25+调节性T淋巴细胞比例的变化及意义

1. 南京市第二医院中西医结合科
2. 南京中医药大学第一临床医学院

基金项目:

江苏省中医药局科技项目(LZ09097)；江苏省中医药领军人才项目(LJ200922)；

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中图分类号: R512.62
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出版历程
- 出版日期: 2012-03-20

The changes and value of the proportion of CD4+CD25+ regulatory T cells in HBeAg (+) and HBeAg (-) ASC in peripheral blood

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摘要

摘要: 目的探讨HBeAg（+）与HBeAg（-）HBV携带者外周血中CD4+CD25+调节性T淋巴细胞（Treg）的变化及其意义。方法 HBeAg（+）与HBeAg（-）HBV携带者两组各50例患者,应用流式细胞仪测定外周血CD3+T淋巴细胞所占比例、CD4+和CD8+T淋巴细胞所占比例及其比值、CD4+CD25+Treg所占比例;以同期门诊健康体检者20例为健康对照组。结果与健康对照组相比,HBeAg（+）与HBeAg（-）HBV携带者,在外周血CD3+T淋巴细胞、CD4+与CD8+T淋巴细胞所占比例差异无统计学意义。CD4+与CD8+T淋巴细胞比值,在HBeAg（+）HBV携带者有升高趋势,但与HBeAg（-）HBV携带者和对照组比较差异无统计学意义。HBeAg（+）HBV携带者,CD4+CD25+Treg所占比例较对照组明显升高,而HBeAg（-）HBV携带者,CD4+CD25+Treg所占比例较对照组明显降低,差异均有统计学意义。结论尽管肝功能在正常范围,HBeAg（+）与HBeAg（-）HBV携带者外周血CD4+CD25+Treg比例不同,HBeAg（+）HBV携带者高于健康对照组...
- 肝炎,乙型,慢性 /
- T淋巴细胞,调节性 /
- 肝炎e抗原,乙型
Abstract: Objective To investigate the Changes and Value of the Proportion of CD4+CD25+regulatory T cells (Treg) in HBeAg (+) and HBeAg (-) chronic asymptomatic hepatitis B virus carriers (ASC) .Methods Patients with HBeAg (+) ASC (n=50) , HBeAg (-) ASC (n=50) and normal subjects (n=20) were recruited in this study.Flow cytometric analysis was used to detect the quantification of peripheral CD3+, CD4+ and CD8+T lymphocyte subgroup, CD4+CD25+Treg.Results The proportion of CD3+, CD4+, CD8+T lymphocyte subgroup has no significant difference among healthy controls, HBeAg (+) and HBeAg (-) ASC.The ratio of CD4 and CD8 in HBeAg (+) patients was elevated, but there was no significant difference between healthy controls and HBeAg (+) patients.Contrast to normal subjects, the ratio of CD4+CD25+Treg increases in HBeAg (+) ASC, but decreases obviously in HBeAg (-) ones (P<0.05) .Conclusion Although having normal hepatic function, HBeAg (+) and HBeAg (-) ASC have different ratio of peripheral CD4+CD25+Treg, Our findings suggest that HBeAg (+) and HBeAg (-) ASC stand in different immune state.
- hepatitis B /
- chronic /
- T-lymphocytes

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参考文献(12)

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