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Study on the expression of HLA-DR/CD69 on CD4+/CD8+T cells in the treatment of HBeAg-positive chronic hepatitis B with interferon alfa-2b
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摘要: 目的观察HBeAg阳性慢性乙型肝炎患者干扰素α-2b治疗前、中、后期CD4+/CD8+T细胞的活性与疗效的相关性。方法 32例患者隔日1次干扰素5 MU肌注,共24周。抽取干扰素α-2b治疗前,治疗1、4、12、24周的外周静脉血,密度梯度离心法分离获得外周血单个核细胞（PBMC）,流式细胞仪检测CD4+/CD8+T细胞比例及CD4+/CD8+T细胞表面CD69、HLA-DR的表达。结果干扰素α-2b治疗结束,HBV DNA含量转阴者12例（37%）,其中HBeAg、抗-HBe血清转换5例（16%）;12例治疗无效（37%）及8例HBV DNA抑制（25%）。HBV DNA转阴者治疗前,治疗1、4周时CD69、HLA-DR表达率较治疗无效及HBV DNA抑制者高,差异有统计学意义（P<0.05）。HBV DNA转阴者治疗后1周时,CD69、HLA-DR表达率最高;4周时CD4+/CD8+T细胞的CD69、HLA-DR表达率较前下降,但仍保持较高表达率;12周时CD69、HLA-DR表达率较前更下降,与治疗无效及HBV DNA抑制者相比差异无统计学意义（P﹥0.05）;24周时CD...Abstract: Objective To study the efficacy prediction and the activation of CD4+/CD8+T cells in the treatment of HBeAg-positive chronic hepatitis B with interferon alfa-2b.Methods 32 patients were given interferon alfa-2b 5 MU every other day for 24 weeks.PBMC were isolated from fresh peripheral blood of chronic severe hepatitis B patients by Ficoll-Hypaque density gradient centrifugation and cultured with plastic-adherence method.FACS was used to analyze the expression of the CD4+/CD8+T cells surface markers, including human leukocyte antigen-DR (HLA-DR) and CD69.Results At the end of treatment, HBV-DNA levels loss rates were 37% (12 cases) , including HBeAg seroconversion in 5 cases (16%) .In 8 cases (25%) HBV-DNA levels were decreased and 12 cases (37%) were ineffective.Although interferon alfa-2b had no effects on the ratio of CD4+/CD8+T cells in chronic severe hepatitis B patients, the expression of CD4+/CD8+T cells activators, such as CD69 and HLA-DR were upregulated, especially in negative HBV-DNA cases.At 1, 4, 12, 24 weeks, there was significant difference (P< 0.05) .Compared with the decreased and ineffective cases, CD69/HLA-DR expression rates in negative HBV-DNA cases were the lowest at 24 weeks (P< 0.05) .Conclusion Interferon alfa-2b can Up-regulate the expression of CD69/HLA-DR on CD4+/CD8+T cells.The activation of CD4+/CD8+T cells levels can predict the efficacy in the treatment of HBeAg-positive chronic hepatitis B with interferon alfa-2b.
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Key words:
- interferon alfa-2b /
- hepatitis B /
- chronic /
- hepatitis B e antigens /
- T-Lymphocytes /
- CD 69
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