Abstract: Objective To summarize the clinical features of Gilbert syndrome (GS) and to improve the diagnosis and treatment level.Methods The family history, clinical manifestations, laboratory examinations and pathological changes of hepatic tissues in 96 patients with GS diagnosed by liver biopsy were analyzed.Results The ratio of male to female is 85∶ 11;6.1% of GS patients have definite family history and 70% of them fall ill between 10-30 years old.The main symptoms of GS are skin and sclera yellow or dark urine and fatigue.Liver function test shows that total bilirubin is (50.5±18.24) μmol/L and the unconjugated bilirubin is (36.19±16.86) μmol/L.Total bilirubin of 97% patients are less than 85.5μmol/L and alanine aminotransferase (ALT) levels of 90% patients are normal.Liver pathological changes are mild with hepatocytes dropsy and denaturation, bile pigments depositing in the hepatocytes and inflammatory cells invading into the liver.Ultrasound visualize that the resonance of livers are coarse and poorly-distributed in 37 paients, splenomegaly and chronic hepatic lesion are shown in 30 and 23 patients respectively.The phenobarbital treatment is more effective than genera treatment.Conclusion GS attacks mainly adolescents, most of them are male.The levels of total bilirubin in GS patients increase less than 5 times upper limit of normal and unconjugated bilirubin rise mainly.Liver pathological changes of GS patients are mild.Phenobarbital is effective.