土三七水煎剂所致肝小静脉闭塞病大鼠模型的建立

宋宇; 禹铮; 樊艳华; 姚树坤; 王泰龄

土三七水煎剂所致肝小静脉闭塞病大鼠模型的建立

1. 中日友好医院消化科
2. 中日友好医院病理科

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中图分类号: R-332;R595.3
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出版历程
- 出版日期: 2011-08-20

A rat model of hepatic veno-occlusive disease induced by the Gynura root water decoction

摘要

摘要: 目的通过观察不同浓度土三七水煎剂所致的大鼠肝脏病理学变化,建立一种模拟临床患者服药所致肝小静脉闭塞病（HVOD）的动物模型。方法土三七2100 g水煎制成浓度为1.5 g/ml土三七生药煎剂,SD大鼠76只随机分为A、B、C、D四组,A、B、C组每组20只,分别以土三七水煎剂高（15 g.kg-1.d-1）、中（7.5 g.kg-1.d-1）、低（3.75 g.kg-1.d-1）浓度灌胃,D组16只以温开水灌胃作为对照,各组大鼠雌、雄各半。每日测量大鼠体重并记录异常表现,灌胃1周和2周后各组大鼠分别处死各半,肝脏标本行病理检查,HE及Masson染色,光镜下参照Deleve评分标准判定病变及严重程度。结果土三七高、中、低浓度组分别有83.3%（15/18）、75.0%（15/20）、40.0%（5/20）出现了肝小静脉闭塞病病理表现;相同剂量下雌性较雄性更易发病,雌、雄鼠造模成功率分别为79.3%（23/29）和41.3%（12/29）（P=0.003）。结论土三七水煎剂灌胃可以成功建立HVOD大鼠模型,剂量和大鼠性别与造模成功率直接相关。
- 疾病模型,动物 /
- 肝静脉闭塞性疾病 /
- 土三七
Abstract: Objective To study the pathological lesions of rat liver induced by the Gynura root water decoction, and establish a rat model for hepatic veno-occlusive disease (HVOD) .Methods 2100 gram Gynura root was prepared into the water decoction with the concentration of 1.5 g/ml.Group A, B and C with 20 SD rats were perfused intragastrically with the Gynura root water decoction with different concentrations of 15 g·kg-1·d-1, 7.5 g·kg-1·d-1 and 3.75 g·kg-1·d-1, respectively.Group D was perfused intragastrically with water which served as the controls.The ratio of male to female was 1:1 in every group.The body weights and symptoms were observed every day.Half of the rats from each group were killed and their liver samples were stained with H&E and Masson techniques after one week and two weeks of drug administration respectively.Results The pathological manifestations of HVOD were observed in the 15 rats of 18 (83.3%) in group A, 15 of 20 (75.0%) in group B and 5 of 20 (40.0%) in group C.With the same dose, the female rats were prone to HVOD.After re-grouping by gender, pathological manifestations of HVOD could be found in 23 of the 29 male rats and 12 of the 29 female rats (79.3% vs 41.3%, P=0.003) .Conclusion The rat HVOD model could be induced by the Gynura root water decoction successfully which was associated with the concentration and gender.
- disease models /
- animal /
- hepatic veno-occlusive disease

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参考文献(15)

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