Determination of the collagen proportionate area of hepatic tissue in HBV-related decompensated cirrhosis using digital image analysis
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摘要: 目的为了了解失代偿期肝硬化肝组织纤维面积比例,本研究采用数字图像分析定量测定因失代偿期乙型肝炎肝硬化行肝移植手术切除的病肝组织纤维化面积比例,以期了解失代偿期肝硬化肝组织纤维化程度。方法 53例因失代偿期慢性乙型肝炎肝硬化行肝移植手术切除的病肝组织,常规石蜡包埋、切片,并对肝组织标本行Masson染色,采用数字图像分析的方法,定量测定肝组织纤维化面积(CPA)。结果失代偿期肝硬化CPA均值为(35.93±14.42)%(11.24%~63.41%),同为失代偿期肝硬化,CPA可相差5倍以上。结论失代偿期肝硬化纤维化面积比例明显增加,CPA最高为63.41%。肝组织纤维化的程度与分布均是肝硬化的重要影响因素。
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关键词:
- 肝炎,乙型,慢性 /
- 肝硬化 /
- 图像解释,计算机辅助
Abstract: Objectives digital image analysis was used to quantitatively determine the collagen proportionate area (CPA) of resected hepatic tissue after liver transplantation due to chronic hepatitis B-induced decompensated cirrhosis.Methods A total of 53 hepatic tissue samples resected from liver transplant patients with chronic hepatitis B-induced decompensated cirrhosis were collected.Masson stains of the hepatic tissue were performed.Digital image analysis was used to quantitatively determine the collagen proportionate area of the hepatic tissue.Results The mean collagen proportionate area at the decompensated cirrhosis stage was (35.93±14.42) % (11.24%-63.41%) .However, the collagen proportionate area of hepatic fibrosis varied up to fivefold at decompensated cirrhotic stage.Conclusion The collagen proportionate area at the decompensated cirrhosis stage was significantly increased, the maximum collagen proportionate area was 63.41%.The density and distribution of fibrosis are also important factors of cirrhosis.-
Key words:
- hepatitis B /
- chronic /
- liver cirrhosis
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[1]Das K, Datta S, Pal S, et al.Course of disease and survival afteronset of decompensation in hepatitis B virus-related cirrhosis[J].Liver Int, 2010, 30 (7) :1033-1042.[Epub 2010 May 14] [2]Chu CM, Liaw YF.Hepatitis B virus-related cirrhosis:naturalhistory and treatment[J].Semin Liver Dis, 2006, 26 (2) :142-152. [3]Mani H, Kleiner DE.Liver biopsy findings in chronic hepatitis B[J].Hepatology, 2009, 49 (5 Suppl) :S61-71. [4]Ishak K, Baptista A, Bianchi L, et al.Histological grading andstaging of chronic hepatitis[J].J Hepatol, 1995, 22 (6) :696-699. [5]Knodell RG, Ishak KG, Black WC, et al.Formulation and applica-tion of a numerical scoring system for assessing histological activityin asymptomatic chronic active hepatitis[J].Hepatology, 1981, 1 (5) :431-435. [6]Scheur PJ, Davies SE, Dhillon AP.Histopathological aspects of vi-ral hepatitis[J].J Viral Hepat, 1996, 3 (6) :277-283. [7]Wright M, Thursz M, Pullen R.Quantitative versus morphologicalassessment of liver fibrosis:semi-quantitative scores are more ro-bust than digital image fibrosis area estimation[J].Liver Int, 2003, 23 (1) :28-34. [8]Arima M, Terao H, Kashima K, et al.Regression of liver fibrosisin cases of chronic liver disease type C:quantitative evaluation byusing computed image analysis[J].Intern Med, 2004, 43 (10) :902-910. [9]O’Brien MJ, Keating NM, Elderiny S, et al.An assessment of dig-ital Image analysis to measure fibrosis in liver biopsy specimens ofpatients with chronic hepatitis C[J].Am J Clin Pathol, 2000, 114 (5) :712-718. [10]Masseroli M, Caballero T, O’Valle F, et al.Automatic quantificationof liver fibrosis design and validation of a new image analysis method:comparison with semiquantitative indexes of fibrosis[J].J Hepatol, 2000, 32 (3) :453-464. [11]Dahab GM, Kheriza MM, El-Beltagi HM, et al.Digital quantificationof fibrosis in liver biopsy sections:description of a new method by pho-toshop software[J].J Gastroenterol Hepatol, 2004, 19 (1) :78-85. [12]Liaw YF, Leung N, Guan R, et al.Asian-Pacific consensus state-ment on the management of chronic hepatitis B[J].Liver Int, 2005, 25 (3) :472-489. [13]Kamath PS, Wiesner RH, Malinchoc M, et al.A model to predictsurvival in patients with end-stage liver disease[J].Hepatology, 2001, 33 (2) :464-470. [14]谢仕斌, 姚集鲁, 郑树森, 等.血清肝纤维化指标水平与肝组织纤维图像分析的关系[J].中华肝脏病杂志, 2002, 8 (4) :203-205. [15]Standish R.An appraisal of the histopathological assesment of liverfibrosis[J].Gut, 2006, 55 (4) :569-578. [16]Calvaruso V, Burroughs AK, Standish R, et al.Computer-assis-ted image analysis of liver collagen:relationship to Ishak scoringand hepatic venous pressure gradient[J].Hepatology, 2009, 49 (4) :1236-1244.
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