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Detection of HBV-LP and relationships between HBV DNA and its level in HBV patients with different patterns of HBV Markers
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摘要: 目的通过检测乙型肝炎病毒标志物（HBV M:HBsAg/抗-HBs、HBeAg/抗-HBe、抗-HBc）不同模式的乙型肝炎患者血清中乙型肝炎病毒外膜大蛋白（HBV-LP）、乙型肝炎病毒前S1抗原（HBVpreS1）、HBV DNA,探讨HBV-LP与HBV DNA及HBV M模式间的关系,研究HBV-LP用于乙型肝炎患者临床诊治的意义。方法采用酶联免疫吸附试验（ELISA）对HBV-LP、HBVpreS1和HBV M进行检测;采用荧光定量PCR方法对HBV DNA进行检测。结果乙型肝炎患者大蛋白检测结果与HBV DNA检测结果差异无统计学意义（χ2=1.97,P>0.05）。HBV-LP A值随HBV DNA拷贝数的增加呈上升趋势,二者之间存在良好的相关性（r=0.565,P<0.01）,在不同HBV DNA拷贝数组别间,HBV-LP A值差异有统计学意义（F=9.23,P<0.01）。结论 HBV-LP是反应HBV感染者体内病毒复制程度的良好的血清免疫学指标,血清中的HBV-LP与HBV DNA具有较好的相关性,特别是可作为HBeAg阴性患者体内监测病毒复制及预后判...Abstract: Objective To investigate the relationship between HBV large envelop protein (HBV-LP) and HBV DNA/patterns of HBV markers and the significances of HBV-LP in clinical diagnosis and treatment of HBV patients, the HBV-LP, preS1 and HBV DNA in HBV patients with different patterns of HBV markers (HBV M, HBsAg/HBsAb、HBeAg/HBeAb、HBcAb) were tested.Methods The HBV-LP, preS1 and patterns of HBV M were tested by ELISA, and HBV DNA was measured by fluorescence quantitative PCR.Results No significant difference was found between HBV-LP and HBV DNA level (χ2=1.97, P>0.05) .There was a positive correlation with the value A of HBV-LP and the HBV DNA level (r=0.565, P<0.01) , and there was a significant difference of value A of HBV-LP in different copies of HBV DNA (F=9.23, P<0.01) .Conclusion The HBV-LP was a good sero-immunological marker to reflect the level of virus replication in HBV patients.The HBV-LP in serum has a good positive correlation with HBV DNA, and especially served as a good serum target in monitoring the virus replication and prognosis.
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Key words:
- hepatitis B /
- hepatitis B virus /
- DNA
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[1]Sinha S, Kumar M.Pregnancy and chronic hepatitis B virus infection[J].Hepatology Research, 2010, 40 (1) :31-48. [2]Bruns M, Miska S, Chassot S, et al.Enhancement of hepatitis Bvirus infection by noninfectious subviral particles[J].J Virol, 1998, 72 (2) :1462-1468 [3]中华医学会.病毒性肝炎防治方案[J].中华传染病杂志, 2001, 19 (1) :56-62 [4]Lambert C, Mann S, Prange R.Assessment of determinants affect-ing the dual topology of hepadnaviral large envelope proteins[J].JGen Virol, 2004, 85 (pt5) :1221-1225. [5]Hildt E, Munz B, Saher G, et al.The preS2 activator MHBst ofhepatitis B virus activates c-raf-1/Erk2 signaling in transgenicmice[J].EMBO J, 2002, 21 (4) :525-535. [6]Foo NC, Ahn BY, Ma X, et al.Cellular vacuolization and apopto-sis induced by hepatitis B virus large surface protein[J].Hepatolo-gy, 2002, 36 (6) :1400-1407. [7]彭建明, 李金明.病毒样颗粒在免疫测定中的应用研究进展[J].中华检验医学杂志, 2005, 28 (2) :214-216. [8]王念跃, 张岱, 葛彦文, 等.血清乙型肝炎病毒大蛋白检测及其临床意义[J].中华实验和临床病毒学杂志, 2010, 24 (5) :349-351. [9]戴利成, 王翠妮, 何健方, 等.血清乙型肝炎病毒外膜大蛋白检测及其与病毒复制的关系[J].中华实验和临床病毒学杂志, 2006, 20 (3) :235-237. [10]Chan HL, Wong VW, Tse AM, et al.Serum hepatitis B surface an-tigen quantitation can reflect hepatitis B virus in the liver and pre-dict treatment response[J].Clin Gastroenlerol Hepatol, 2007, 5 (12) :1462-1468. [11]魏红山, 黄玉波, 宋淑静, 等.e抗原阴性慢性乙型肝炎患者血清表面抗原大蛋白水平与乙型肝炎病毒DNA之间的关系[J].中华肝脏病杂志, 2006, 14 (7) :543-544. [12]黄国永, 陈筱华, 王海虹, 等.乙型肝炎病毒大蛋白 (LHBs) 研究进展[J].中华实验和病毒学杂志, 2008, 22 (3) :236-237. [13]吴正林, 庄鹏, 林汉利, 等.乙型肝炎病毒表面大蛋白检测用于筛查隐匿性HBV感染[J].中华实验和病毒学杂志, 2009, 23 (5) :381-383. -
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