The clinical significance of the analysis of gene amplification and protein expression of α-smooth muscle actin in the liver of patients with CHB
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摘要: 目的研究慢性乙型肝炎(CHB)肝活检组织α-肌动蛋白(α-SMA)的表达,探讨α-SMA在慢性乙型肝炎病毒感染中的作用及意义。方法通过RT-PCR对CHB活检组织的α-SMA进行半定量,结合肝脏组织学和免疫组化,对CHB患者的肝组织α-SMA的表达进行分析。结果在基因水平上,轻度CHB患者α-SMA/β-actin为0.126±0.032,与中度(0.323±0.099,P=0.001)和重度(0.410±0.103,P=0.000)比较差异有统计学意义;但在CHB患者中,中度和重度比较差异无统计学意义(P=0.097)。免疫组化显示:在CHB肝组织,α-SMA主要与坏死性炎症反应有关,贮脂细胞和肝窦内皮细胞表达α-SMA较强。CHB轻度组(G1/G2),α-SMA表达于坏死性炎症反应部位;CHB较重组(G3/G4),则表达于坏死区及其相邻的肝实质表达明显增加,且Kuppfer细胞明显增多。结论 HBV慢性感染状态下,α-SMA的表达上调,可能参与了与肝脏患者的CHB病理过程。Abstract: Objective To investigate the roles and significance of α-smooth muscle actin (α-SMA) expression in liver biopsies of patients with CHB.237 liver biopsy tissues were used to observe the correlation between severity of CHB and the level of expression of α-smooth muscle actin.Methods The expression of α-SMA in the liver biopsies from patients with CHB were semi-quantified by RT-PCR and analysis was performed recombinant with histological and immunochemical methods.Results The expression of α-SMA varied in different liver biopsies.In the mRNA level, the expression quantity of α-SMA in G1/G2 group was 0.126±0.032, in G2/G3 groups was 0.323±0.099 (P=0.001) and in the G3/G4 groups was 0.410±0.103 (P=0.000) .There was significant difference among them.Moreover, in the protein levels, expression of α-SMA in G1/G2 groups was located in the necrosis and inflammation sites.In the G3/G4 groups they were very seriously in the liver Disses space tissues and stellate cells and there were more Kuffer's cells involved in the regions.Conclusion Expression of α-SMA were up-regulated in CHB liver biopsies whether in the mRNA levels or in the protein levels.And their expression and distribution may be involved in CHB pathologic course, which may be a new concept involved in chronic HBV infection progress.
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Key words:
- hepatitis B /
- chronic /
- actin /
- gene amplification
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