慢性乙型肝炎肝组织α-肌动蛋白基因扩增及蛋白表达分析的临床意义

邵先安; 严家春; 谷旻; 马勇; 李俊峰; 徐长江

慢性乙型肝炎肝组织α-肌动蛋白基因扩增及蛋白表达分析的临床意义

解放军123医院检验病理科

基金项目:

南京军区卫生专业人才培养“122工程”资助项目(2007-039)；

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中图分类号: R512.62
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出版历程
- 出版日期: 2011-05-20

The clinical significance of the analysis of gene amplification and protein expression of α-smooth muscle actin in the liver of patients with CHB

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摘要

摘要: 目的研究慢性乙型肝炎（CHB）肝活检组织α-肌动蛋白（α-SMA）的表达,探讨α-SMA在慢性乙型肝炎病毒感染中的作用及意义。方法通过RT-PCR对CHB活检组织的α-SMA进行半定量,结合肝脏组织学和免疫组化,对CHB患者的肝组织α-SMA的表达进行分析。结果在基因水平上,轻度CHB患者α-SMA/β-actin为0.126±0.032,与中度（0.323±0.099,P=0.001）和重度（0.410±0.103,P=0.000）比较差异有统计学意义;但在CHB患者中,中度和重度比较差异无统计学意义（P=0.097）。免疫组化显示:在CHB肝组织,α-SMA主要与坏死性炎症反应有关,贮脂细胞和肝窦内皮细胞表达α-SMA较强。CHB轻度组（G1/G2）,α-SMA表达于坏死性炎症反应部位;CHB较重组（G3/G4）,则表达于坏死区及其相邻的肝实质表达明显增加,且Kuppfer细胞明显增多。结论 HBV慢性感染状态下,α-SMA的表达上调,可能参与了与肝脏患者的CHB病理过程。
- 肝炎,乙型,慢性 /
- 肌动蛋白类 /
- 基因扩增 /
- 蛋白质生物合成
Abstract: Objective To investigate the roles and significance of α-smooth muscle actin (α-SMA) expression in liver biopsies of patients with CHB.237 liver biopsy tissues were used to observe the correlation between severity of CHB and the level of expression of α-smooth muscle actin.Methods The expression of α-SMA in the liver biopsies from patients with CHB were semi-quantified by RT-PCR and analysis was performed recombinant with histological and immunochemical methods.Results The expression of α-SMA varied in different liver biopsies.In the mRNA level, the expression quantity of α-SMA in G1/G2 group was 0.126±0.032, in G2/G3 groups was 0.323±0.099 (P=0.001) and in the G3/G4 groups was 0.410±0.103 (P=0.000) .There was significant difference among them.Moreover, in the protein levels, expression of α-SMA in G1/G2 groups was located in the necrosis and inflammation sites.In the G3/G4 groups they were very seriously in the liver Disses space tissues and stellate cells and there were more Kuffer's cells involved in the regions.Conclusion Expression of α-SMA were up-regulated in CHB liver biopsies whether in the mRNA levels or in the protein levels.And their expression and distribution may be involved in CHB pathologic course, which may be a new concept involved in chronic HBV infection progress.
- hepatitis B /
- chronic /
- actin /
- gene amplification

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参考文献(16)

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