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Plkl与p21WAF1/CIP1在肝细胞癌中的表达

李辉 张婷 鲁凤民 徐春晖 唐芙爱 陈香梅 马军

引用本文:
Citation:

Plkl与p21WAF1/CIP1在肝细胞癌中的表达

基金项目: 

国家自然科学基金资助项目(30771099); “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项“十一五”计划项目(2009ZX10004-903); 

详细信息
  • 中图分类号: R735.7

Associated expression of Plkl and p21WAF1/CIP1 in hepatocellular carcinoma

Research funding: 

 

  • 摘要: 目的细胞周期抑制因子p21WAF1/CIP1(p21)可在转录水平抑制Polo-like kinase1(Plk1)基因的表达,本文旨在探讨Plk1和p21蛋白在肝细胞癌中的表达及相关性。方法应用Western Blot方法检测10对原发性肝细胞癌和癌旁组织中Plk1和p21蛋白的表达情况。通过将pFlex-p21表达质粒瞬时转染人肝癌细胞系HepG2细胞,进一步检测p21过表达对Plk1mRNA和蛋白表达的影响。结果 Plk1在10例肝癌组织中的表达均高于配对癌旁组织,p21蛋白在7对肝癌组织中的表达高于配对癌旁组织。HepG2细胞瞬时表达p21质粒后,Plk1的蛋白表达和mRNA水平均无变化(P>0.05)。结论 Plk1和p21在肝细胞癌组织中的表达具有一定的肿瘤特异性。肝癌组织中p21蛋白过表达可能不具有抑制Plk1表达的作用,具体机制还有待于进一步研究。

     

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  • 出版日期:  2011-04-20
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