PGE2,mPGES-1和HBx在肝细胞癌的表达及相关性

王燕; 王晓茜; 李秀金; 唐南洪

PGE2,mPGES-1和HBx在肝细胞癌的表达及相关性

福建医科大学附属协和医院省肝胆外科研究所

基金项目:

卫生部吴阶平医学基金会肝病实验诊断研究基金(LDWMF-SY-2011B008)；

详细信息

中图分类号: R735.7
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出版历程
- 出版日期: 2011-02-20

Correlation between hepatocellular carcinoma and the expressions of prostaglandin E2, mPGES-1 and HBx

Research funding:

摘要

摘要: 目的研究肝细胞癌（HCC）患者的前列腺素E2（PGE2）、微粒体型前列腺素E2合成酶-1（mPGES-1）与乙型肝炎病毒X蛋白（HBx）的表达情况、相关性和临床意义。方法采用酶联免疫法检测65例HCC患者外周血和癌组织的PGE2水平,用免疫组织化学法检测癌组织及癌旁肝组织mPGES-1和HBx的表达,并分析这些指标与患者的临床病理特征的关系。结果肝癌组织PGE2含量明显高于癌旁肝组织,分别为（9.10±2.03）ng/g和（2.78±0.19）ng/g;肝癌患者外周血中PGE2含量为（128.74±14.44）pg/ml,与肝癌组织中PGE2含量呈正相关（r=0.862）;HCC组织中HBx和mPGES-1的阳性表达率分别为86.2%和78.5%,而癌旁肝组织的HBx和mPGES-1阳性表达率分别为81.5%和46.2%,其中mPGES-1蛋白在肝癌组织及癌旁肝组织的阳性表达率差异有统计学意义（P<0.05）。HBx和mPGES-1的表达升高与肝癌分化程度和肝外是否转移相关（P<0.05）;HBx的表达与mPGES-1的表达呈正相关（r=0.389）;外周血PGE2水平和组...
- 癌,肝细胞 /
- 地诺前列酮 /
- 前列腺素内过氧化物合酶类 /
- 病毒蛋白质类 /
- 肝炎病毒,乙型
Abstract: Objective To assess the correlation between prostaglandinE2 levels, the expression of hepatitis B virus X protein and microsomal PGE2 synthase-1 in patients with hepatocellular carcinoma, and to explore their clinical significance.Methods The PGE2 levels in peripheral blood and carcinoma tissues were measured by a kit of PGE2 ELISA.Expression of HBx and mPGES-1 proteins in HCC and adjacent tissues were detected by immunohistochemistry, then their correlation with clinicopathological features was assayed.Results The contents of PGE2 were significantly higher in the tissues of HCC (9.10±2.03) ng/g than those in tumor margins of excision (2.78±0.19) ng/g.The contents of PGE2 in peripheral blood were correlated with those in the HCC tissues (r=0.862) .Immunohistochemical staining results revealed that the positive expression of HBx and mPGES-1 were 86.2% and 78.5% in tumor tissues, 81.5% and 46.2% in margins of excision, respectively.The expression of mPGES-1 in tumor tissues and margins of excision were statistically significant (P<0.05) .The increased expression of HBx and mPGES-1 correlated with differentiation and hepatic metastasis.The expression of HBx and mPGES-1 in HCC tissues were in positive correlation (r=0.389) .The PGE2 levels in peripheral blood and expression of mPGES-1 in patients with HCC were enhanced, indicating a positive correlation of the levels in two samples (r=0.526) .Conclusion PGE2, HBx and mPGES-1 are involved in the processes of invasion and metastasis of HCC.PGE2 levels in peripheral blood might be used to evaluate the metastasis of HCC.HBx and mPGES-1 might be a potential new targets for HCC.
- carcinoma /
- hepatocellular /
- dinoprostone /
- prostaglandin-endoperoxide synthases /
- viral Proteins

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参考文献(15)

[1]Ushio A, Takikawa Y, Lin SD, et al.Induction of Bcl-xL is a possible mechanism of anti-apoptotic effectby prostaglandin E2 EP4-receptor agonist in humanhepatocellular carcinoma HepG2 cells[J].Hepatol Res, 2004, 29 (3) :173-179.

[2]Murakami M, Naraba H, Tanioka T, et al.Regulation ofprostaglandin E2 biosynthesis by inducible membrane-associated prostaglandin E2 synthase that acts inconcert with cyclooxy genase-2[J].Biol Chem, 2000, 275 (42) :32783-32792.

[3]Takii Y, Abiru S, Fujioka H, et al.Expression of microso-mal prostaglandin E synthase-1 in human hepatocelluarcarcinoma[J].Liver Int, 2007, 27 (7) :989-996.

[4]Lara-Pezzi E, Gómez-Gaviro MV, Gálvez BG, et al.Thehepatitis B virus X protein promotes tumor cell invasionby inducing membrane-type matrix metalloproteinase-1and cyclooxygenase-2 expression[J].Clin Invest, 2002, 110 (12) :1831-1838.

[5]Cavallaro U, Christofori G.Cell adhesion and signallingby cadherins and Ig-CAMs in can-cer[J].Nat RevCancer, 2004, 4 (2) :118-132.

[6]Park EJ, Lee JH, Yu GY, et al.Dietary and geneticobesity promote liver inflammation and tumorigenesisby enhancing IL-6 and TNF expression[J].Cell, 2010, 140 (2) :197-208.

[7]Tang JY, Aszterbaum M, Athar M, et al.Basal cell car-cinoma chemoprevention with nonsteroidal anti-inflammatory drugs in genetically predisposed PTCH1+/-humans and mice[J].Cancer Prev Res, 2010, 3 (1) :25-34.

[8]Marusawa H, Matsuzawa S, Welsh K, et al.HBXIPfunctions as a cofactor of survivin in apopt-osissuppression[J].EMBO, 2003, 22 (11) :2729-2740.

[9]Koga T, Shibahara K, Kabashima A, et al.Overexpressionof cyclooxygenase-2 and tumor angiogenesis in humangastric cancer[J].Hepatogastroenterology, 2004, 51 (60) :1626-1630.

[10]Han R, Tsui S, Smith TJ.Up-regulation of prostaglandinE2 synthesis by interleukin-1beta in human orbitalfibroblasts involves coordinate induction of prostaglandin-endoperoxide H synthase-2 and glutathione-dependentprostaglandin E2 synthase expression[J].Biol Chem, 2002, 277 (19) :16355-16364.

[11]Fuson AL, Komlosi P, Unlap TM, et al.Immunolocalization ofa microsomal prostaglandin E synthase in rabbit kidney[J].Am J Physiol Renal Physiol, 2003, 285 (3) :558-564.

[12]Tavernarakis N, Wang SL, Dorovkov M, et al.Heritableand inducible genetic interference by double-strandedRNA encoded by transgenes[J].Nat Genet, 2000, 24 (2) :180-183.

[13]Cheng AS, Chan HL, Leung WK, et al.Expressionof HBx and COX-2 in chronic hepatitis B, cirrhosisand hepatocellular carcinoma:implication of HBX inupregulation of COX-2[J].Modern Pathology, 2004, 17 (10) :1169-1179.

[14]Kamei D, Murakami M, Nakatani Y, et al.Potentialrole of microsomal prostaglandin E synthase-1 intumorigenesis[J].Biol Chem, 2003, 278 (21) :19396-19405.

[15]Zweifel BS, Davis TW, Ornberg RL, et al.Direct evidencefor a role of cyclooxygenase2 drived prostaglandin E2 inhuman head and neck xenograft tumors[J].Cancer Res, 2002, 62 (22) :6706-6711.

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