Correlation between the recent thymic output function and the frequency of nave T cell in CHB patients
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摘要: 目的了解慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)中T细胞受体重排删除环(TRECs)含量和PBMCs中初始T细胞比例,分析两者的相关关系。方法采集CHB患者和健康人外周静脉血标本,(1)应用实时荧光定量聚合酶链反应(RT-PCR)技术检测TRECs含量;(2)利用荧光标记抗体FITC-CD3、PE-CD45RA、PE-cy5-CD62L标记细胞表面分子,流式细胞仪检测PBMCs中CD3+CD45RA+CD62L+细胞比例;(3)TRECs含量与初始T细胞相关性分析。结果 (1)CHB组TRECs含量为(6.78±1.06)拷贝/103 PBMCs,低于对照组(8.16±2.07)拷贝/103 PBMCs,P<0.01。(2)CHB组初始T细胞比例为(20.73±6.75)%,低于对照组(27.82±9.66)%,P<0.05。(3)CHB组初始T细胞比例与TRECs含量呈正直线相关,相关系数r=0.688,P<0.01。对照组初始T细胞比例与TRECs含量正相关,r=0.646,P<0.01。两组r值比较差异无统计学意义,P>0.05。结论...Abstract: Objective To test the content of T cell receptor excision circles (TRECs) and the frequency of naive T lymphocytes in patients with chronic HBV infection and analyze their correlation.Methods Peripheral blood were collected from normal individuals and patients with chronic HBV infections. (1) TRECs were assessed by real-time PCR; (2) Lymphocytes were labeled with fluorescent-labeled antibodies FITC-CD3, PE-CD45RA and PE-cy5-CD62L and flow cytometry was used to detect the expressions of CD3, CD45RA and CD62L on the surface of T lymphocytes.Results (1) The TRECs (6.78±1.06) copies/103 in patients (n=25) with chronic HBV infections was significantly lower than that (8.16±2.07) copies/103 in healthy controls (n=25) (P<0.01) .The frequency of nave T cells (20.73±6.75) % in patients (n=25) with chronic HBV infections was significantly lower than that (27.82±9.66) % in healthy controls (n=25) (P<0.05) . (2) Both in patients with chronic HBV infections and in healthy controls, the frequency of nave T cells were in positive linear correlation with the TRECs, while the coefficient correlation were 0.688 (P<0.01) and 0.646 (P<0.01) respectively.The coefficient correlation in the two groups had no statistical differences, (P>0.05) .Conclusion Both the nave T cell in peripheral blood and the TRECs in patients with chronic HBV infection were decreased compare to the healthy adult, and the two indices had better linear correlation.
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Key words:
- hepatitis B /
- chronic /
- receptors /
- antigen
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