家系感染乙型肝炎病毒基因变异与HLA-Ⅱ类等位基因的关系

谢德胜; 张清波; 陈常兴; 黄建芳; 马瑨; 黄海滨; 度尧

家系感染乙型肝炎病毒基因变异与HLA-Ⅱ类等位基因的关系

1. 复旦大学附属上海市第五人民医院
2. 复旦大学附属华山医院

基金项目:

上海市闵行自然科学研究项目(2007MH020)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2010-04-20

The relationship between HBV gene mutation in HBV infected family and HLA class Ⅱ alleles

Research funding:

摘要

摘要: 目的研究家系内慢性乙型肝炎病毒基因变异特征及与HLA-II类分子基因多态性的关系。方法采用PCR扩增和产物测序检测HBV前C区1896位及BCP区1762/1764位变异,分析家系内慢性HBV感染者121例（实验组）病毒变异特征及与非家系慢性乙肝患者83例（对照组）病毒变异频率差异;用PCR/SSP对家系内慢性HBV感染者的HLA-DQA1和DQB1等位基因多态性进行测定;分析HLA-II类分子基因多态性与家系内慢性乙型肝炎病毒基因变异的关系。结果实验组前C区1896位变异、BCP区1762/1764位双变异及联合变异率均高于对照组;HLA-DQA1*0501和DQB1*0301等位基因频率和家系慢性HBV感染者前C区1896位、BCP区1762/1764位及联合变异有关;母亲为第一代的感染家系中,子女的前C区1896位及BCP区1762/1764位变异与母亲是否有该位点变异差异无显著性。结论家系感染HBV患者中,HBV前C区1896位、BCP区1762/1764位双突变及联合变异频率明显高于无家系感染的慢性乙型肝炎患者;家系内感染HBV前C区1896位、BCP区1762和1764位变...
- 肝炎病毒,乙型 /
- HLA-DQ抗原 /
- 多态现象,遗传 /
- 等位基因
Abstract: Objective To study the relationship between the characteristics of mutation in hepatitis B virus (HBV) infected family and HLA class II molecular genetic polymorphism.Methods The mutations of HBV pre-Core area 1896 and the basal core promoter (BCP) 1762/1764 were amplified and sequenced.The characteristics and frequencies of the two site mutations of HBV were analyzed in 121cases of infected family (experimental group) and 83 cases with chronic hepatitis B (control group) of non-infected family.The HLA-DQA1 and DQB1 alleles were tested using PCR/SSP.The relationship between the HLA-II polymorphism in (HBV) infected family and HBV gene mutation was analyzed.Results The variations of pre-Core area 1896, and BCP area 1762/1764 in experimental group were higher than the control group.Combination mutations in HLA-DQA1*0501and DQB1*0301 alleles, and HBV pre-Core area 1896 and BCP1762/1764 were found in the experimental group.In the mothers of HBV infected families as the first generation, the mutations in HBV pre-Core area 1896 and BCP1762/1764 of the offspring has no relationship.Conclusion The pre-Core area 1896 and BCP area 1762/1764 mutations of HBV combined with HLA-DQA1*0501 and DQB1*0301 frequencies in family infection cases were significantly higher than those patients without family infection.There may be a relationship between the pre-Core area 1896 and BCP area 1762/1764 mutations and the immune status
- hepatitis B virus /
- HLA-DQ antigens /
- polymorphism /
- genetici

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参考文献(11)

[1]Ashraf M, 1 Masoud S, 1 Scott B, et al.Downregulation of HLAClass II Molecules by G1896APre-Core Mutation in Chronic Hep-atitis B Virus Infection[J].Viral Immunology, 2009, 22 (5) ∶295-300.

[2]中华医学会.慢性乙型肝炎防治指南[J].中华肝脏病杂志, 2005, 13 (12) ∶881-891.

[3]Thio CL, Carrington M, Marti D, et al.Class II HLA alleles andhepatitis B virus persistence in African Americans[J].J InfectDis, 1999, 179 (4) ∶1004-1006.

[4]杨友国, 刘成永, 王春颖, 等.家族聚集性慢性乙型肝炎的病毒前C区和基本核心启动子变异与临床病理的关系[J].世界华人消化杂志, 2008, 16 (26) ∶2997-3000.

[5]Truong BX, Seo Y, Yano Y, et al.Genotype and variations in corepromoter and pre-core regions are related to progression of diseasein HBV-infected patients from Nothern Vietnam[J].Int J MolMed, 2007, 19 (2) ∶293-299.

[6]Shen T, Yan MX, Zou YL, et al.Virologic characteristics of hep-atitis B virus in patients infected via maternal-fetaltransmission[J].World J Gastroenterol, 2008, 14 (37) ∶5674-5682.

[7]Xu YY, Yu JY, Zhong YW, et al.Association between the fre-quency of class II HLAantigens and the susceptibility to intrauterineinfection of hepatitis B virus[J].J Biol Sci, 2008, 4 (2) ∶111-115.

[8]Baas A, Gao X, Chelvanavanam G.Peptide binding motifsand spe-cificities for HLA-DQ molecules[J].Immunogenetics, 1999, 50 (1/2) ∶8-15.

[9]Jung MC, Pape GR.Immunology of hepatitis B infection[J].Lan-cet Infect Dis, 2002, 2 (1) ∶43-50.

[10]Chang JJ, Lewin SR.Immunopathogenesis of hepatitis B virus in-fection[J].Immunol Cell Biol, 2007, 85 (1) ∶16-23.

[11]Tai PC, Banik D, Lin GI, et al.Novel and frequent mutations ofhepatitis B virus coincide with a major histocompatibility complexclass I-restricted T-cell epitope of the surface antigen[J].J Vir-ol, 1997, 71 (6) ∶4852-4856.

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