家系感染乙型肝炎病毒基因变异与HLA-Ⅱ类等位基因的关系

谢德胜; 张清波; 陈常兴; 黄建芳; 马瑨; 黄海滨; 度尧

家系感染乙型肝炎病毒基因变异与HLA-Ⅱ类等位基因的关系

1. 复旦大学附属上海市第五人民医院
2. 复旦大学附属华山医院

基金项目:

上海市闵行自然科学研究项目(2007MH020)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2010-04-20

The relationship between HBV gene mutation in HBV infected family and HLA class Ⅱ alleles

Research funding:

摘要

摘要: 目的研究家系内慢性乙型肝炎病毒基因变异特征及与HLA-II类分子基因多态性的关系。方法采用PCR扩增和产物测序检测HBV前C区1896位及BCP区1762/1764位变异,分析家系内慢性HBV感染者121例（实验组）病毒变异特征及与非家系慢性乙肝患者83例（对照组）病毒变异频率差异;用PCR/SSP对家系内慢性HBV感染者的HLA-DQA1和DQB1等位基因多态性进行测定;分析HLA-II类分子基因多态性与家系内慢性乙型肝炎病毒基因变异的关系。结果实验组前C区1896位变异、BCP区1762/1764位双变异及联合变异率均高于对照组;HLA-DQA1*0501和DQB1*0301等位基因频率和家系慢性HBV感染者前C区1896位、BCP区1762/1764位及联合变异有关;母亲为第一代的感染家系中,子女的前C区1896位及BCP区1762/1764位变异与母亲是否有该位点变异差异无显著性。结论家系感染HBV患者中,HBV前C区1896位、BCP区1762/1764位双突变及联合变异频率明显高于无家系感染的慢性乙型肝炎患者;家系内感染HBV前C区1896位、BCP区1762和1764位变...
- 肝炎病毒,乙型 /
- HLA-DQ抗原 /
- 多态现象,遗传 /
- 等位基因
Abstract: Objective To study the relationship between the characteristics of mutation in hepatitis B virus (HBV) infected family and HLA class II molecular genetic polymorphism.Methods The mutations of HBV pre-Core area 1896 and the basal core promoter (BCP) 1762/1764 were amplified and sequenced.The characteristics and frequencies of the two site mutations of HBV were analyzed in 121cases of infected family (experimental group) and 83 cases with chronic hepatitis B (control group) of non-infected family.The HLA-DQA1 and DQB1 alleles were tested using PCR/SSP.The relationship between the HLA-II polymorphism in (HBV) infected family and HBV gene mutation was analyzed.Results The variations of pre-Core area 1896, and BCP area 1762/1764 in experimental group were higher than the control group.Combination mutations in HLA-DQA1*0501and DQB1*0301 alleles, and HBV pre-Core area 1896 and BCP1762/1764 were found in the experimental group.In the mothers of HBV infected families as the first generation, the mutations in HBV pre-Core area 1896 and BCP1762/1764 of the offspring has no relationship.Conclusion The pre-Core area 1896 and BCP area 1762/1764 mutations of HBV combined with HLA-DQA1*0501 and DQB1*0301 frequencies in family infection cases were significantly higher than those patients without family infection.There may be a relationship between the pre-Core area 1896 and BCP area 1762/1764 mutations and the immune status
- hepatitis B virus /
- HLA-DQ antigens /
- polymorphism /
- genetici

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参考文献(11)

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