Abstract: Objective To study the effect of ultraviolet-blue ray and resin adsorption on bilirubin clearance, and develop a new method to purify plasma from artificial liver treatment. Methods The patients were classified in two groups. In Group A patients the plasma from artificial liver treatment was alkalified to pH 10 (plasma: 0.2mol/L Na2CO3=1∶1, V/V) , irradiated by the ultraviolet-blue ray for 10-14 min and then filtrated by a non-polar adsorbent resin for 3 min (D4006, crosslinked polysyrene) . In Group B, The plasma was alkalified to pH 10 (plasma: 2mol/L Na2CO3=1∶1, V/V) , irradiated by the ultraviolet-blue ray for 15 min and then filtrated by the D4006 resin for 5 minute to decrease the level of bilirubin.Results In group A patients, the clearance rates of total, direct and indirect bilirubin (TBil, DBil, IBil) and the recovery rate of ALB were 86%, 84%, 87.5% and 99% by irradiation of alkalified plasma, and 85%, 84%, 86% and 98% by irradiation then adsorption of alkalified plasma. In group B, the clearance rates of total, direct and indirect bilirubin (TBil, DBil, IBil) , and the recovery rate of ALB were 85%, 84%, 86% and 99% by irradiation of alkalified plasma and 90%, 87%, 91% and 90% by irradiation then adsorption of alkalified plasma. Conclusion The levels of bilirubin decreased significantly by the ultraviolet-blue ray irradiaton on the alkalified plasma, and the level also decreased further by the irradiation then adsorption by the D4006 resin. This new method may be useful to purify plasma from artificial liver treatment.plasma exchange; bilirubin; resins, synthetic; adsorption; ultraviolet therapy; liver, artificial