纤维化分期对大鼠肝组织血管紧张素Ⅱ AT1受体表达的影响

袁成民; 张纵; 吴谙诏; 吕卉; 岳凤娥

纤维化分期对大鼠肝组织血管紧张素Ⅱ AT1受体表达的影响

济南市传染病医院

基金项目:

济南市科技发展基金资助课题(编号:056017)；

详细信息

中图分类号: R575.2
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出版历程
- 出版日期: 2010-01-20

The effect of Fibrosis stages on the expression of Angiotensin Ⅱ type 1 (AT1) receptor

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摘要

摘要: 目的探讨肝组织中血管紧张素Ⅱ1型受体的表达与四氯化碳诱导大鼠肝纤维化分期的相关性。方法肝组织HE染色检测病理改变,免疫组化技术检测血管紧张素Ⅱ AT1受体在肝组织的表达,应用计算机进行灰度扫描,采用德国Leica公司生产的Qwiuv图像分析软件测定每一样本的灰度值,根据纤维化不同分期的灰度值进行统计学处理。结果随着大鼠肝纤维化加重,血管紧张素Ⅱ AT1受体在肝组织的表达增多,经统计学处理各期之间差异有统计学意义。结论纤维化分期与大鼠肝组织血管紧张素Ⅱ AT1受体的表达有明显的相关性。
- 纤维化 /
- 大鼠肝脏 /
- 血管紧张素Ⅱ受体
Abstract: Objective To study the relationship between the levels of angiotensin Ⅱ type 1 (AT1) receptor and the fibrosis stages in the rats afoter induced by CCl4.Methods The pathology of liver was evaluated by Hematoxylin and Eosin (HE) staining and the levels of AT1 receptors were measured by immunochemistry staining.The gray values of the samples were measured by Qwiuv picture analysis software of the Leica Company (Germany) .Results The degree of fibrosis had greatly increased along with the increase in AT1 receptor level in the rat liver.Conclusion There is a significant correlation between the stages of fibrosis and the expression of AT1 receptor.
- fibrosis /
- liver of the Rat /
- angiotensin Ⅱ type 1 (AT1) receptor

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参考文献(11)

[1]龚作炯, 宋仕玲, 黄砚青, 等.血管紧张素ⅡAT1受体拮抗剂对大鼠肝纤维化的疗效及作用机制[J].肝脏, 2004, 2 (9) 97～100.

[2]王卫卫, 杨希山, 吴平生, 等.纤维化及正常人肝组织中血管紧张素ⅡAT1受体mRNA表达[J].第一军医大学学报, 2003, 5 (23) 477～478.

[3]魏红山, 李定国, 陆汉明, 等.血管紧张素受体阻断剂抗肝纤维化的实验研究[J].中华肝脏病杂志, 2000, 8 (5) ∶302～304.

[4]赵景民.Ito细胞在大鼠实验性肝硬变中的动态研究[J].中华病理学杂志, 1991, 20 (2) ∶91.

[5]Bataller R, Gabele E, Parsons CJ, et al.Systemic infusion of angioten-sinⅡexacerbates liver fibrosis in bile duct-ligated rats[J].Hepatol-ogy, 2005, 41 (5) ∶1046-1055.

[6]Bataller R, Schwabe RF, choi YH, et al.NADPH oxidase signal transduces angiotensinⅡin hepatic stellagte cells and is critical in hepatic fibrosis[J].J Clin lnvest, 2003, 112 (9) ∶1383-1394.

[7]Liu J, Gong H, Zhang ZT, et al.Effect of angiotensinⅡand angioten-sinⅡtype1receptor antagonist on the proliferation, contraction and collagen synthesis in rat hepatic stellate cells[J].Chin Med J (En-gl) , 2008, 121 (2) ∶161-165.

[8]李旭, 杨希山, 吴平生, 等.肝星形细胞表达醛固酮合成酶基因及安体舒通抗肝纤维化的研究[J].中华肝脏病杂志, 2001, 9 (2) 75～77.

[9]Mifune M, Sasamura H, Shimiru H, et al.AngiotensinⅡtype2recep-tors stimulate collagen synthesis in cultured vascular smooth musle cells[J].Hypertension, 2000, 36 (50) ∶845-50.

[10]Yoshiji H, Kuriyama S, Yoshiji J, et al.AngiotensinⅡtype1recep-tor integrations is a major regulator for liver febrosis development in rats[J].Hepatology, 2001, 34 (4) ∶745-50.

[11]Yang XS, Li X, Wu PS, et al.CYP11B2expression in rat liver and the effect of spironolactone on hepatic fibrogenesis[J].Horm Res, 2000, 33 (6) ∶288-93.

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