聚集素、P糖蛋白在肝细胞癌中的表达及其相关性研究

林照亮; 熊茂明; 钱福永; 吴炎; 李红春; 王玉梅

聚集素、P糖蛋白在肝细胞癌中的表达及其相关性研究

1. 深圳市西丽人民医院消化内科
2. 安徽医科大学第一附属医院肝胆外科
3. 深圳市东湖医院外科
4. 深圳市第六人民医院消化内科

基金项目:

深圳市南山区科技计划项目〔2006035)；

详细信息

中图分类号: R735.7
计量
- 文章访问数: 2910
- HTML全文浏览量: 6
- PDF下载量: 800
- 被引次数: 0
出版历程
- 出版日期: 2009-05-20

Research funding:

摘要

摘要: 目的探讨聚集素（Clusterin）蛋白在肝细胞癌（HCC）中的表达及其与P糖蛋白（P-glycoprotein,P-gp）表达的相关性。方法采用免疫组化S-P法检测凋亡抑制基因Clusterin在HCC、肝硬化和正常肝组织中的表达及P-gp蛋白在HCC中的表达。结果Clusterin蛋白在HCC中的阳性表达率为82.93%,在肝硬化和正常肝组织中表达阴性或弱阳性;Clusterin蛋白阳性表达与患者的年龄、性别及肝硬化无关,与Edmenson组织学分级有相关性。P-gp蛋白在肝癌组织中的阳性表达率为70.73%。在HCC中,Clusterin蛋白的表达与P-gp蛋白表达呈正相关。结论Clusterin蛋白在HCC中呈高表达,与临床耐药密切相关,有望成为肝癌靶向治疗的一个新靶点。
- 肝细胞癌 /
- 聚集素蛋白 /
- P糖蛋白 /
- 免疫组织化学
Abstract: Objective To study the expression of clusterin protein and its relationship with expression of P-glycoprotein in human hepatocellular carcinoma (HCC) .Methods The expression of clusterin gene in HCC, cirrhosis and normal tissue and the expression of P-glycoprotein in HCC were detected by S-P immunohistochenical method.Results The positive expression rate of clusterin protein was 82.93% in HCC, and negative or weak in cirrhosis and normal liver tissue.The positive expression rate of clusterin protein was significantly correlated with Edmondson's histological grade, but was no correlated with patients'age, sex and with or without cirrhosis.The positive expression rate of P-glycoprotein in HCC were 70.73%.There was a significant correlation between the expression of clusterin protein and P-glycoprotein.Conclusion The high expression rate of clusterin is significantly associated with clinical multidrug resistance in HCC.It could be a potential target for treatment of HCC.
- human hepatocellular carcinoma /
- clusterin protein /
- P-glycoprotein /
- immunohistochemistry

HTML全文

参考文献(13)

[1]Trougakos IP, Gonos ES.Clusterin/apolipoprotein J in human aging and cancer[J].Int J Biochem Cell Biol, 2002, 34:1430-1448.

[2]Rosenberg ME, Silkensen J.Clusterin:Physiologic and pathophysio-logic considerations[J].Int J Biochem Cell Biol, 1995, 27:633-645.

[3]Steinberg J, Oyasu R, Lang S.Intracellular levels of SGP-2 (clus-terin) correlate with tumor grade in prostate cancer[J].Clin Cancer Res, 1997, 3:1707-1711.

[4]Redondo M, Villar E, Torres-Munoz J.Overexpression of clusterin in human breast carcinoma[J].Am J Pathol, 2000, 157:393-399.

[5]Miyake H, Hara S, Arakawa S.Overexpression of clusterin is an in-dependent prognostic factor for nonpapillary renal cell carcinoma[J].J Urol, 2002, 167:703-706.

[6]Miyake H, Gleave M, Kamidono S.Overexpression of clusterin in transitional cell carcinoma of the bladder is related to disease progres-sion and recurrence[J].Urology, 2002, 59:150-154.

[7]XIE Min-Jue, MOTOO Yoshiharu, SU Shi-Bing.Expression of clusterin in human pancreatic cancer[J].Pancreas, 2002, 25:234-238.

[8]Chen X, Halberg RB, Ehrhardt WM.Clusterin as a biomarker in mu-rine and human intestinal neoplasia[J].Proc Natl Acad Sci USA, 2003, 100:9530-9535.

[9]Wellmann A, Thieblemont C, Pittaluga S.Detection of differentially expressed genes in lymphomas using cDNA arrays:Identification of clusterin as a new diagnostic marker for anaplastic large-cell lym-phomas[J].Blood, 2000, 96:398-404.

[10]YUN Kyung-kang, SE0NG Woo-hong, Hyucksang Lee, et al.Overexpression of clusterin in human hepatocellular carcinoma[J].Hum Pathol, 2004, 35 (11) :1340-1346.

[11]周利群, 杨学贞, 曾荔, 等.聚集素在前列腺正常、增生、癌组织中的表达及意义[J].中华外科杂志, 2003, 41 (2) :93-95.

[12]熊茂明, 区庆嘉, 高劲松, 等.肝癌多药耐药基因表达的临床研究[J].中华普通外科杂志, 2003, 18 (6) :364-366.

[13]Sensibar JA, Sutkowski DM, Raffo A, et al.Prevention of cell death induced by tumor necrosis factor alpha in LNCaP cells by overexpres-sion of sulfated glycoprotein-2 (clusterin) [J].Cancer Res, 1995, 55 (11) :2431-2437.

施引文献

资源附件(0)

访问统计