慢性乙型肝炎患者外周血单个核细胞Toll样受体4的表达及其与TGF-β的关系

魏丽; 邓永东; 陈红

慢性乙型肝炎患者外周血单个核细胞Toll样受体4的表达及其与TGF-β的关系

兰州大学第一医院感染科

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中图分类号: R512.62
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出版历程
- 出版日期: 2009-05-20

摘要

摘要: 目的了解慢性乙型肝炎、肝硬化患者外周血单个核细胞（PBMC）中Toll样受体4（TLR4）的表达变化及其意义。方法采用实时荧光定量RT-PCR方法分别检测28例慢性乙型肝炎、25例肝硬化及10例健康对照者PBMC中TLR4mRNA的表达;ELISA法检测上述患者血清转化生长因子β（TGF-β）;放射免疫法检测血清透明质酸（HA）、层粘蛋白（LN）、Ⅲ型前胶原蛋白（PCⅢ）及Ⅳ型胶原（CⅣ）的水平。结果各实验组TLR4mRNA的表达均明显高于健康对照组（P<0.01）,且与患者血清TGF-β表达水平呈显著正相关（r值=0.875,P<0.01）,与HA、LN、PCⅢ、CⅣ表达水平之间呈正相关（r值分别为0.647、0.525、0.556、0.682）。结论慢性乙型肝炎患者外周血单个核细胞TLR4的表达增高可能与肝纤维化的形成密切相关。
- 肝炎,乙型,慢性 /
- 受体,Toll /
- 转化生长因子β /
- 实时荧光定量RT-PCR
Abstract: Objective To examine the expression of Toll-like receptor (TLR) 4 mRNA in the peripheral blood mononuclear cells (PBMC) from patients with hepatitis B virus (HBV) infection and explore the relationship between TLR4 and chronic HBV infection.Methods the expression level of TLR4 were determined by Quantitative real-time PCR respectively in PBMC from 28 chronic hepatitis B patients, 25 liver cirrhosis patients and 10 healthy controls.The level of serum TGF-β was detected by ELISA.Meanwhile, HA、 LN、 PCⅢ and CⅣ was measured.Results The expression level of TLR4 in patients were higher than those in healthy controls (P<0.01) .And the TLR4 expression level was significantly positively correlated with serum TGF-β level (r=0.875, P<0.01) , as well as the level of HA、 LN、 PCⅢ and CⅣ (r=0.647、0.525、0.556、0.682, respectively) .Conclusion the upregulated expression of TLR4 in patients with chronic HBV infection were significantly related with the degree of liver fibrosis and the development of the disease.
- hepatitis B /
- chronic /
- receptors /
- Toll-like

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参考文献(9)

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