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摘要: 目的探讨HBVC基因型较B基因型易引起肝炎慢性化的机制。方法从GenBank分别下载C基因型和B基因型小S蛋白的氨基酸序列(即HBsAg),用CLustalW2.0生物软件分别比较C基因型和B基因型小S蛋白氨基酸序列的相似性;用神经网络的方法预测同基因型不同的小S蛋白氨基酸序列之间的T细胞表位的差异。结果通过分别比较C基因型和B基因型小S蛋白的氨基酸序列的相似性,C基因型小S蛋白的氨基酸序列之间的相似性为(90～100)%,B基因型小S蛋白的氨基酸序列之间的相似性为(94～100)%;同基因型不同的小S蛋白氨基酸序列之间的T细胞表位存在明显差异。结论C基因型小S蛋白的氨基酸序列之间较大的变异和同基因型不同的小S蛋白氨基酸序列之间的T细胞表位的差异可能是HBVC基因型较B基因型易引起肝炎慢性化的原因或部分原因。Abstract: Objective To discuss the mechanism of C genotype HBV bing easier to arose chronic hepatitis than B genotype HBV.Methods Download amino acid sequences of HBV C genotype small S protein and HBV B genotype small S protein and analyze comparability of these amino acid sequences by software CLustalW 2.0.To forecast the differerce of T cell epitope of different small S protein sequences of C genotype HBV using NN way.Results The result of comparability of small S protein of B genotype HBV and C genotype HBV showed that comparability of amino acid sequence of small S protein of B genotype HBV is 94～100%, and C genotype HBV is 90～100%;the result that genotype is same but small S protein sequence is different results in different T cell epitope.Conclusion The cause or part cause of C genotype HBV bing easier to arose chronic hepatitis than B genotype HBV can be that variation of small S protein sequence of C genotype HBV is bigger than B genotype, the bigger variation result in higher variation ratio of T cell epitope.
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Key words:
- HBV /
- genotype /
- T cell epitope /
- small S protein
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[1]周建良, 吴诗品.乙型肝炎病毒基因型与病毒复制的关系[J].临床肝胆病杂志, 2003, 19 (50) :286-287. [2]许军, 王齐欣, 蒋栋, 等.乙型肝炎病毒基因型与病情轻重的关系[J].中华肝脏病杂志, 2003, 11 (1) :11-13. [3]骆利敏, 李明, 夏虎, 等.乙型肝炎病毒多表位抗原基因的设计、合成及表达[J].中华微生物学和免疫学杂志, 2004, 24 (6) :426-432. -
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