HBV基因型(B/C)是影响HBeAg阳性慢性乙型肝炎患者血清HBsAg水平的重要因素

陈金军; 王战会; 马世武; 周彬; 侯金林; 温淑娟

HBV基因型(B/C)是影响HBeAg阳性慢性乙型肝炎患者血清HBsAg水平的重要因素

1. 南方医科大学南方医院感染内科肝病中心
2. 南方医科大学南方医院检验医学中心

基金项目:

国家自然科学基金(30671873)；

详细信息

中图分类号: R512.62;R446.6
计量
- 文章访问数: 2062
- HTML全文浏览量: 13
- PDF下载量: 861
- 被引次数: 0
出版历程
- 出版日期: 2009-04-20

Research funding:

摘要

摘要: 目的了解HBV基因型（HBV/B和HBV/C）对血清HBsAg水平的影响。方法对492例慢乙型肝炎患者应用亚培12000进行血清HBsAg定量检测,基因分型采用PCR联合限制性片段长度多态性方法。将不同基因型HBV复制质粒转染Huh7细胞,比较HBsAg分泌效率。结果HBV/B患者（n=260）血清HBsAg水平高于HBV/C（n=197）患者（P<0.001）。以全部患者为研究对象的多变量回归分析发现,HBV DNA水平（B=0.098,P=0.002）、HBeAg状态（B=0.594,P<0.001）和HBV基因型（B=-0.420,P<0.001）决定血清HBsAg水平;在HBeAg（+）患者中（n=337）,HBV DNA水平（B=0.136,P=0.001）、性别（B=0.441,P=0.011）和HBV基因型（B=-0.278,P=0.049）决定血清HBsAg水平;而在HBeAg（-）患者中（n=155）,仅HBV DNA水平（B=0.195,P=0.018）是影响血清HBsAg水平的因素。转染试验表明HBV/C的HBsAg的分泌效率仅有HBV/B的1/...
- 慢性乙型肝炎 /
- HBsAg /
- 基因型 /
- 乙型肝炎病毒
Abstract: Objective To investigate the impacts of HBV genotypes (HBV/B and HBV/C) on serum HBsAg levels in chronic hepatitis B patients.Methods Quantification of serum HBsAg was performed with Abbott 12000 and HBV genotypes were determined by PCR-RFLP in 492 patients.Replicative plasmids contained common genotype HBV genome was transiently transfected into Huh7 cells and HBsAg secretion profile was compared.Results Serum HBsAg levels in patients infected with HBV/B (n=260) were higher (P<0.001) than those in HBV/C (n=197) .Multivariate regression analysis with all 492 subjects showed that HBV DNA levels (B=0.098.P=0.002) .HBeAg status (B=0.594.P<0.001) and HBV genotype (B=-0.420.P<0.001) had significant influences on serum HBsAg levels.Regression analysis in HBeAg (+) patients (n=337) showed that HBV DNA (B=0.136, P=0.001) .gender (B=0.441.P=0.011) and HBV genotype (B=-0.278, P=0.049) determined serum HBsAg levels.however.in HBeAg (-) patients (n=155) HNV DNA (B=0.195.P=0.018) was the only factor influenced serum HBsAg levels.HBsAg sectetion efficiency was 33% in HBV/C transfection to that in HBV/B (P=0.010) .Conclusion HBV genotypes affected serum HBsAg levels through different HBsAg secretion efficiency in HBeAg chronic hepatitis B patients.
- chronic hepatitis B /
- hepatitis B surface antigen /
- genotype /
- hepatitisB virus

HTML全文

参考文献(10)

[1]Yim HJ, Lok AS.Natural history of chronic hepatitis B virus infec-tion:what we knewin1981 and what we knowin2005[J].Hepatol-ogy, 2006, 43 (2 Suppl 1) ∶S173-S181.

[2]陈金军, 马世武, 王战会, 等.乙型肝炎病毒基因型B/Cg型患者血清和肝细胞内HBsAg表达水平的差异[J].中华医学杂志, 2006, 86∶1947-1951.

[3]Zeng GB, Wen SJ.Wang ZH.et al.A novel hepatitis B virus genoty-ping system by using restriction fragment length polymorphism pat-terns of S gene amplicons[J].World J Gastroenterol, 2004, 10 (21) ∶3132-3136.

[4]Sugiyama M, Tanaka Y, Kato T, et al.lnfluence of hepatitis B virusgenotypes on the intraand extracellular expression of viral DNA andantigens[J].Hepatology, 2006, 44 (4) ∶915-924.

[5]Chan HL, Hui AY, Wong ML, et al.Genotype C hepatitis B virus in-fection is associated with an increased risk of hepatocellular carcino-ma[J].Gut, 2004, 53 (10) ∶1494-1498.

[6]Chu CJ, Hussain M, Lok AS.Hepatitis B virus genotype B is associ-ated with earlier HBeAg seroconversion compared with hepatitis B vi-rus genotype C[J].Gastroenterology, 2002, 122 (7) ∶1756-1762.

[7]Furusyo N, Nakashima H, Kashiwagi K, et al, Clinical outcomes ofhepatitis B virus (HBV) genotypes B and C in Japanese patients withchronic HBV infection[J].Am J Trop Med Hyg, 2002, 67 (2) ∶151-157.

[8]Kao JH, Chen PJ, Lai MY, et al.Hepatitis B genotypes correlate withclinical outcomes in patients with chronic hepatitis B[J].Gastroen-terology, 2000, 118 (3) ∶554-559.

[9]Milich D, Liang TJ.Exploring the biological basis of hepatitis B e an-tigen in hepatitis B virus infection[J].Hepatology, 2003, 38 (5) ∶1075-1086.

[10]陈金军, 王战会, 马世武, 等.乙型肝炎病毒基因型对拉米夫定治疗早期血清乙型肝炎表面抗原水平变化的影响[J].肝脏, 2008, 13 (2) ∶101-103.

施引文献

资源附件(0)

访问统计