HBV基因型(B/C)是影响HBeAg阳性慢性乙型肝炎患者血清HBsAg水平的重要因素

陈金军; 王战会; 马世武; 周彬; 侯金林; 温淑娟

HBV基因型(B/C)是影响HBeAg阳性慢性乙型肝炎患者血清HBsAg水平的重要因素

1. 南方医科大学南方医院感染内科肝病中心
2. 南方医科大学南方医院检验医学中心

基金项目:

国家自然科学基金(30671873)；

详细信息

中图分类号: R512.62;R446.6
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出版历程
- 出版日期: 2009-04-20

Research funding:

摘要

摘要: 目的了解HBV基因型（HBV/B和HBV/C）对血清HBsAg水平的影响。方法对492例慢乙型肝炎患者应用亚培12000进行血清HBsAg定量检测,基因分型采用PCR联合限制性片段长度多态性方法。将不同基因型HBV复制质粒转染Huh7细胞,比较HBsAg分泌效率。结果HBV/B患者（n=260）血清HBsAg水平高于HBV/C（n=197）患者（P<0.001）。以全部患者为研究对象的多变量回归分析发现,HBV DNA水平（B=0.098,P=0.002）、HBeAg状态（B=0.594,P<0.001）和HBV基因型（B=-0.420,P<0.001）决定血清HBsAg水平;在HBeAg（+）患者中（n=337）,HBV DNA水平（B=0.136,P=0.001）、性别（B=0.441,P=0.011）和HBV基因型（B=-0.278,P=0.049）决定血清HBsAg水平;而在HBeAg（-）患者中（n=155）,仅HBV DNA水平（B=0.195,P=0.018）是影响血清HBsAg水平的因素。转染试验表明HBV/C的HBsAg的分泌效率仅有HBV/B的1/...
- 慢性乙型肝炎 /
- HBsAg /
- 基因型 /
- 乙型肝炎病毒
Abstract: Objective To investigate the impacts of HBV genotypes (HBV/B and HBV/C) on serum HBsAg levels in chronic hepatitis B patients.Methods Quantification of serum HBsAg was performed with Abbott 12000 and HBV genotypes were determined by PCR-RFLP in 492 patients.Replicative plasmids contained common genotype HBV genome was transiently transfected into Huh7 cells and HBsAg secretion profile was compared.Results Serum HBsAg levels in patients infected with HBV/B (n=260) were higher (P<0.001) than those in HBV/C (n=197) .Multivariate regression analysis with all 492 subjects showed that HBV DNA levels (B=0.098.P=0.002) .HBeAg status (B=0.594.P<0.001) and HBV genotype (B=-0.420.P<0.001) had significant influences on serum HBsAg levels.Regression analysis in HBeAg (+) patients (n=337) showed that HBV DNA (B=0.136, P=0.001) .gender (B=0.441.P=0.011) and HBV genotype (B=-0.278, P=0.049) determined serum HBsAg levels.however.in HBeAg (-) patients (n=155) HNV DNA (B=0.195.P=0.018) was the only factor influenced serum HBsAg levels.HBsAg sectetion efficiency was 33% in HBV/C transfection to that in HBV/B (P=0.010) .Conclusion HBV genotypes affected serum HBsAg levels through different HBsAg secretion efficiency in HBeAg chronic hepatitis B patients.
- chronic hepatitis B /
- hepatitis B surface antigen /
- genotype /
- hepatitisB virus

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参考文献(10)

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