Detection and evaluation of serum proteomic patters for hepatocellular carcinoma treated by interventional therapy Using SELDI-TOF-MS protein chip array
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摘要: 目的用表面增强激光解吸离子化飞行时间质谱(surface-enhanced laser desorption-ionization timeof-flight mass spectrometry,SELDI-TOF-MS)蛋白质芯片检测肝细胞性肝癌(简称肝癌)患者介入治疗前后血清蛋白质组的变化,初步探讨肝癌介入治疗的药物靶点。方法应用SELDI-TOF-MS蛋白质芯片检测50例未经治疗的肝癌患者、50例介入治疗的肝癌患者,获得弱阳离子交换芯片(weak cationic exchanger)蛋白表达图谱,Bio-Marker Wizard软件分析差异蛋白。结果未经治疗的肝癌患者和介入治疗的肝癌患者血清中检测到不同质荷比处有3个差异蛋白质峰,其蛋白质含量差异有统计学意义(P<0.05)。数据库搜索这3个蛋白质峰,得到3种与之分子量最为接近的蛋白质。结论应用SELDI-TOF-MS筛选肝癌患者介入治疗的药物靶点快速、有效,检测到的3个差异蛋白质可能是肝癌患者介入治疗的药物靶点。Abstract: Objective To detect the serum proteomic patters in hepatocellular carcinoma (HCC) patients treated by interpose chemotherapy by using SELDI-TOF-MS (surface-enhanced laser desorption-ionization time-of-flight mass spectrometry) protein chip array technology, screen drug targets, evaluate its clinical significance.Methods 50 cases of the patients with hepatocellular carcinoma without receiving any therapy, 50 cases of the patients with hepatocellular carcinoma receiving the interpose chemotherapy were tested by weak cationic exchanger protein chip and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry.The diagnostic models were developed and validated by BioMarker Wizard.Results At the different M/Z value range, three proteins were obviously different in the group of the patients with hepatocellular carcinoma without receiving any therapy, the patients with hepatocellular carcinoma receiving the interpose chemotherapy.Through searching databases we have got three probably significant proteins related with them.Conclusions Detection of serum proteomic patters for hepatocellular carcinoma treated by interventional therapy using SELDI-TOF-MS protein chip array is quick and effective.These differential proteins could be drug targets of hepatocellular carcinoma before and after the interpose chemotherapy in the serum.
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Key words:
- Hepatocellular Carcinoma /
- Proteomics /
- Drug targets /
- SELDI /
- ProteinChip
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