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肝细胞性肝癌患者介入治疗前后血清蛋白质组的变化

师越 李悦国

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肝细胞性肝癌患者介入治疗前后血清蛋白质组的变化

详细信息
  • 中图分类号: R735.7

Detection and evaluation of serum proteomic patters for hepatocellular carcinoma treated by interventional therapy Using SELDI-TOF-MS protein chip array

  • 摘要: 目的用表面增强激光解吸离子化飞行时间质谱(surface-enhanced laser desorption-ionization timeof-flight mass spectrometry,SELDI-TOF-MS)蛋白质芯片检测肝细胞性肝癌(简称肝癌)患者介入治疗前后血清蛋白质组的变化,初步探讨肝癌介入治疗的药物靶点。方法应用SELDI-TOF-MS蛋白质芯片检测50例未经治疗的肝癌患者、50例介入治疗的肝癌患者,获得弱阳离子交换芯片(weak cationic exchanger)蛋白表达图谱,Bio-Marker Wizard软件分析差异蛋白。结果未经治疗的肝癌患者和介入治疗的肝癌患者血清中检测到不同质荷比处有3个差异蛋白质峰,其蛋白质含量差异有统计学意义(P<0.05)。数据库搜索这3个蛋白质峰,得到3种与之分子量最为接近的蛋白质。结论应用SELDI-TOF-MS筛选肝癌患者介入治疗的药物靶点快速、有效,检测到的3个差异蛋白质可能是肝癌患者介入治疗的药物靶点。

     

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  • 出版日期:  2009-02-20
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