Detection high mobility group box-1 protein in autoimmune hepatitis
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摘要: 目的采用ELISA检测方法,初步探讨人高迁移率族蛋白B1(HMGB1)与自身免疫性肝炎(AIH)发病的相互作用关系。方法将纯化的HMGB1蛋白,包被ELISA检测微孔板,检测AIH患者和健康献血员的血清分别165份和69份,分析比较AIH患者和健康献血员的血清中抗HMGB1抗体的分泌情况。结果AIH患者血清中抗HMGB1抗体的阳性率明显高于健康献血员(78.7%VS50.7%,P<0.05)。直线回归分析显示,抗HMGB1抗体滴度(OD值)与ALT水平有很好的相关性(相关系数r=0.968)。结论HMGB1是AIH疾病发生发展中一个重要的炎症蛋白,与疾病的炎症活动程度有很好的相关性。Abstract: Objective Adaption the method of ELISA, investigation the relationship between the human high mobility group box-1 protein (HMGB1) and autoimmune hepatitis (AIH) .Methods Coating the microwell plate using purified HMGB1 protein, detection the serum of the 165 AIH and 69 healthy respectively.Comparison the level of Anti-HMGB1 antibody in AIH and the healthy.Results The positive ratio of Anti-HMGB1 antibody in AIH more than that in healthy (78.7% VS 50.7%, P<0.05) , The linear regression analysis showed there were good correlation between the anti-HMGB1 antibody titer and the level of alanine transarninase (ALT) (correlation coefficient, r=0.968) .Conclusion HMGB1 is a crucial inflammatory protein in the progressing of AIH, there are good correlation between the anti-HMGB1 antibody titer and the stage of inflammation in AIH.
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