肝脏疾病患者血清细胞因子IFN-γ、TNF-α、IL-4、IL-10的检测

王崇; 姜艳芳; 金清龙; 鲍万国; 王峰; 牛俊奇

肝脏疾病患者血清细胞因子IFN-γ、TNF-α、IL-4、IL-10的检测

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中图分类号: R575;R446.6
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- 出版日期: 2009-02-20

The detection of cytokines IFN-γ、TNF-α、IL-4、IL-10 in the serum of patients of liver disease

摘要

摘要: 目的分析细胞因子TNF-α、IFN-γ、IL-4、IL-10在肝病患者血中的浓度改变及临床意义。方法应用酶联免疫吸附测定（ELISA）试剂盒体外检测不同类型肝病患者血清细胞因子TNF-α、IFN-γ、IL-4、IL-10的浓度。结果IL-4、TNF-α、IFN-γ在急性乙型肝炎、慢性乙型肝炎、乙型肝炎肝硬化及重型肝炎组患者外周血的浓度,较正常对照组,均显著升高（P<0.001～P<0.05）,而IL-10的肝病组与正常组之间、肝病组之间相比,均未有显著差别（P>0.05）;IFN-γ在乙型肝炎肝硬化组中的浓度显著高于慢性乙型肝炎组（P<0.01）和重型肝炎组（P<0.05）;IL-4在重型肝炎组中的浓度显著高于急性（P<0.05）、慢性乙型肝炎组（P<0.05）。结论细胞因子IL-4、IFN-γ、TNF-α在乙型肝炎病毒感染中起重要作用;细胞因子IL-4、TNF-α,IFN-γ分别参与乙型肝炎病毒感染中的促纤维化作用和抗纤维化作用;促炎因子IFN-γ、TNF-α和抗炎因子IL-4共同参与重型肝炎的发病过程。
- 细胞因子 /
- 肝病
Abstract: Objective This study was designed to detect the concentration of cytokines TNF-α、IFN-γ、IL-4、IL-10 and assess its clinical significance in different liver diseases.Methods A blood sample was collected from 165 patients of liver diseases which were admitted to First Hospital of Jilin University from Feb.2006-Feb.2007, among which 36 were severe hepatitis, 54 HBV related cirrhosis, 46 chronic HBV hepatitis, 15 acute HBV hepatitis patients.14 healthy people were chosen as control group.the liver diseases were diagnosed on the basis of《prevention and cure of viral hepatitis》and 《Hepatic failure guideline》.Double sandwiches ELISA was used to detect serum cytokines concentration.Results IFN-γ, TNF-α, IL-4 concentrations in acute and chronic HBV hepatitis, HBV related cirrhosis and severe hepatitis groups were increased significantly than that of normal control group (P<0.05) , while IL-10 showed no difference between liver disease group and normal control group.Among liver cases, IFN-γ concentration in cirrhosis group was significantly higher than that of chronic HBV hepatitis and severe hepatitis group (P<0.05) .IL-4 concentration in severe hepatitis group was significantly higher than that of acute and chronic hepatitis B (P<0.05) .Conclusion Cytokines such as TNF-α, IFN-γ and IL-4 play an important role and involve in the fibrogenesis and anti-fibrogenesis in the HBV infection.Proinflammatory factors of TNF-α, IFN-γ and anti-inflammatory factor of IL-4 work together in the course of severe hepatitis.
- cytokine /
- liver disease

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