丹黄方对部分肝叶切除大鼠肝组织中Tec的影响

朱清静; 杨玲; 蔡艳萍; 盛国光

丹黄方对部分肝叶切除大鼠肝组织中Tec的影响

1. 武汉市医疗救治中心
2. 华中科技大学同济医学院附属协和医院
3. 湖北省中医院

基金项目:

国家中医药管理局中医药科学技术研究专项(No.06-07JP25)；湖北省科技厅科技攻关计划项目(No.2005AA301C12)；

详细信息

中图分类号: R285.5
计量
- 文章访问数: 2376
- HTML全文浏览量: 17
- PDF下载量: 803
- 被引次数: 0
出版历程
- 出版日期: 2009-01-20

Influence of DanHuangFang on expression of Tec mRNA in liver regeneration model with partial liver excision in rats

Research funding:

摘要

摘要: 目的探讨丹黄方对部分肝叶切除大鼠肝组织中Tec mRNA表达的影响。方法采用部分肝叶切除肝再生模型。24只大鼠随机分为假手术组、手术组、丹黄方组和pHGF组。除假手术组外其余三组接受部分肝叶切除手术。从手术前3天至手术后48小时,丹黄方组给与丹黄方灌胃（10g/kg）及腹腔注射生理盐水（4.0ml/kg）,每天一次;促肝细胞生长素（pHGF）组给与生理盐水灌胃（4.0ml/kg）和腹腔注射pHGF（1ml/100g）,每天一次;手术组及假手术组仅给与生理盐水（4.0ml/kg）灌胃和腹腔注射。采用RT-PCR方法检测Tec mRNA的表达。结果丹黄方组肝组织中Tec mRNA的表达显著高于假手术组和手术组,P<0.01;与pHGF组比较无显著差异,P>0.05。结论丹黄方具有促进部分肝叶切除大鼠肝组织中Tec mRNA的表达的作用。
- 丹黄方 /
- 肝再生 /
- 促肝细胞生长素
Abstract: Objective To explore the effect of DanHuangFang (DHF) on the expression of Tec mRNA in the liver regeneration model with partial liver excision in rats.Methods The liver regeneration model with partial liver excision in rat was employed.Twenty four Wistar rats were randomly divided into four groups: the sham operation group, the operation group, the DHF group and the pHGF group.The operation of partial liver excision was carried on in the latter three group rats.From three days before the operation to 48 hours after the operation, the rats in the DHF group were lavaged with DHF (10g/kg) once a day, and intraperitoneally injected with sodium chloride (4.0ml/kg) simultaneously.The rats in the pHGF group were lavaged with sodium chloride (4.0ml/kg) and intraperitoneally injected with pHGF (1ml/100g) .The rats in the sham operation and the operation group were only lavaged and intraperitoneally injected with sodium chloride (4.0ml/kg) .The expression of Tec mRNA was detected by RT-PCR methods.Results The expression of Tec mRNA in DHF group was significantly higher than that of the sham operation and the operation group (P<0.01) .And there was no difference between the DHF group and pHGF group in the expression of HGFmRNA.Conclusion DHF can promote the expression of Tec mRNA in the liver regeneration model with partial liver excision.
- DanHuangFang (DHF) /
- Liver regeneration /
- Tec mRNA

HTML全文

参考文献(9)

[1]Higgins GM, Anderson R M.Experimental pathology of the liver:I.regeneration of the liver by the white rat folling parital suricaironoval[J].Arch Pathol, 1931, 12∶186

[2]Panis Y, Lomri N, Emond JC.Early gene expression associated withregeneration is intact after massive hepatectomy in rats[J].J SurgRes, 1998, 2∶103.

[3]Mano H, Ishikawa F, Nishida J, et al.A novel protein-tyrosinekiease, Tec is preferntially expressed in liver[J].Oncogene, 1990, 5 (12) ∶1781.

[4]Fromm C, Coso OA, Montaner S, et al.The small GTP-bindingprorein links G protein-coupled rceptors and Galphal2 to the serumrespouse element and cellular transfomration[J].Proc Natl Acad SciUSA, 1997, 94 (19) ∶10098.

[5]Pillai S, Stewart T, Moran.TEC kinase pathways in lymphocyte de-velopment and transfomoanon[J].Biophysica Acta, 2002, 1602 (2) ∶162.

[6]汪思应, 卢海妹, 郑红, 等.用差异显示杂交技术筛选与造血相关的新基因[J].安徽医科大学学报, 2000, 35 (6) ∶422-423.

[7]朱清静, 陈锋, 盛国光, 等.大黄对暴发性肝衰竭大鼠肝损伤及肝再生的影响[J].中国中西医结合消化杂志, 2006, 14 (1) ∶5-6.

[8]大黄对大鼠肝再生过程中信号调节蛋白α1影响的实验研究[J].中西医结合肝病杂志, 2006, 16 (1) ∶21-23.

[9]朱清静, 蔡艳萍, 周星, 等。丹黄方对大鼠肝再生过程中PC3、c-fos、LRF-1影响的实验研究[J].中西医结合肝病杂志, 2008, 18 (1) ∶32-34.

施引文献

资源附件(0)

访问统计