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HBV变异患者外周血CD4+CD25+调节性T细胞变化及其临床意义

仇娇敏 韩莹 计云霞 董培玲 尚宏伟 王兴翠 张斌 丁惠国

引用本文:
Citation:

HBV变异患者外周血CD4+CD25+调节性T细胞变化及其临床意义

基金项目: 

北京市教育委员会基金的资助(KM200710025024); 北京市科教计划(H0006246040791); 

详细信息
  • 中图分类号: R512.62

Relationship between CD4+CD25+ regulatory T cells and exacerbation of liver disease related HBV mutation patients

Research funding: 

 

  • 摘要: 目的研究乙型肝炎病毒(HBV)变异患者外周血CD4+CD25+调节性T细胞(Treg细胞)及肝组织CD8+T细胞的关系,并探讨其临床意义。方法慢性乙型肝炎患者64例。HBV变异者27例,无变异者37例;急性乙型肝炎患者16例。各组间年龄、性别无明显差异。25例患者进行肝脏穿刺活检,免疫组化方法检测肝组织CD8+T细胞分布;流式细胞技术测定外周血Treg细胞变化;荧光定量PCR测定血清HBVDNA水平,PCR-RFLP测定前C区G1896A、P区YMDD变异。结果急性乙型肝炎患者、HBV变异伴肝病进展患者外周血Treg细胞比例明显低于HBV变异肝病无明显变化者(3.00±1.33,2.57±0.83vs4.32±0.96,P<0.01),后者与HBV无变异者没有明显差异(4.32±0.96vs4.77±2.11,P>0.05)。外周血Treg细胞与HBVDNA水平呈正相关(r=0.411,P<0.01);免疫组化显示HBV变异伴肝病进展患者肝组织CD8+T明显增多,急性乙型肝炎患者肝组织汇管区较多CD8+T浸润。结论Treg比例降低与HBV变异肝病进展可能有关,可作为H...

     

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  • 出版日期:  2008-06-20
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