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Expression of CD4+CD25+Tr in the peripheral blood of hepatitis B virus in fection and its clinical significance
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摘要: 目的探讨乙肝病毒感染者外周血CD4+CD25+调节性T细胞（CD4+CD25+Tr）表达水平及其临床意义。方法选择乙肝病毒携带者25例、急性乙肝患者26例、慢性乙肝患者38例,26例正常对照,用流式细胞仪检测外周血中CD4+CD25+Tr表达水平;用PCR法检测患者HBV DNA载量。结果乙肝病毒携带者组与慢性乙肝患者组CD4+CD25+Tr水平（9.93±3.51,11.21±3.29）明显高于对照组（8.12±2.80）（P<0.01或0.05）,急性肝炎组与对照组相比,差异没有显著性。结论持续性HBV感染者CD4+CD25+Tr表达增加,提示CD4+CD25+Tr在慢性乙型肝炎中担负着重要的免疫调节作用,可能抑制HBV感染者特异性细胞免疫反应,与乙肝病毒感染的慢性化及肝病的临床发生、发展有关。
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关键词:
- CD4+CD25+调节性T细胞 /
- 乙型肝炎 /
- 流式细胞仪
Abstract: Objective To explore the expression of CD4+CD25+ Tr in the peripheral blood of hepatitis B virus infection and its clinical significance.Methods Selection of asymptomatic carriers (ASC, n=25) acute hepatitis B (AHB, n=26) , chronic hepatitis B (CHB, n=38) and normal subjects (n=26) , CD4+CD25+Tr in peripheral blood of the four groups were detected by flow cytometry, the level of HBVDNA in serum were dynamically detected by PCR.Results CD4+ CD25+Tr expression in ASC and CHB elevated significantly than that in normal subjects (P<0.05 or P<0.01) .However, there is little discrepancy between AHB and normal subjects.Conclusion CD4+CD25+Tr in ASC and CHB is increased.The finding suggested that CD4+CD25+Tr play an active role in heptitis B virus infection, and relate clinical development.-
Key words:
- CD4+CD25+Tr /
- hepatitis B /
- flow cytometry
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