HBeAg阴、阳性慢乙肝患者细胞免疫状态及HBVDNA水平分析

吴健林; 曾志励; 吴继周; 陈茂伟; 玉艳红; 刘志红; 黄璐

HBeAg阴、阳性慢乙肝患者细胞免疫状态及HBVDNA水平分析

1. 广西医科大学第一附属医院
2. 广西医科大学护理学院

基金项目:

广西壮族自治区科技厅自然科学基金资助课题(桂科自0542074)；

详细信息

中图分类号: R512.62
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出版历程
- 出版日期: 2008-04-20

A Comparison of Cellular immune status and HBV DNA Levels in patiens with HBeAgnegative and HBeAg-positive chronic hepatitis B

Research funding:

摘要

摘要: 目的对比HBeAg阴性及HBeAg阳性慢性乙肝患者在细胞免疫状态及HBV DNA水平方面的差别,分析造成这种差别的可能原因。方法应用流式细胞仪进行T淋巴细胞分类检测、荧光定量PCR测量血清中的HBVDNA。结果在HBVDNA水平方面,HBeAg阴性慢性乙肝患者均值为（5.13±1.43）Log,显著低于HBeAg阳性组的（6.29±1.70）Log（P<0.05）;在T淋巴细胞分类方面,HBeAg阴性慢性乙肝患者CD8+T显著高于HBeAg阳性患者,分别是:23.25±3.79和20.69±3.30（P<0.05）,总T、CD4+T及CD44+/CD8+比值方面两组无明显差别（P>0.05）。结论HBeAg阴性慢性乙肝患者HBV DNA水平低于HBeAg阳性患者,可能与HBeAg阴性慢性乙肝患者具有相对较强的细胞免疫状态有关。
- 慢性乙型肝炎 /
- 细胞免疫 /
- HBVDNA水平 /
- T淋巴细胞分类
Abstract: Objective To investigate the possible cause of the difference in cellular immune status and HBVDNA levels in patiens with HBeAg-negative and HBeAg-positive chronic hepatitis B.Methods Peripheral T lymphocyte subsets were examined by flow cytometry, and HBVDNA level was assayed by fluorescent quantitative polym erase chain reaction (FQ-PCR) .Results The level of HBVDNA in HBeAg-negative group was 5.13±1.43 Log, which was lower than that in HBeAg-positive group (6.29±1.70 Log) P<0.05;the quantity of CD8+T in HBeAg-negative group was higher than that in HBeAg-positive group, their values were 23.25±3.79 and 20.69±3.30 respectively (P<0.05) .However, there was no significant difference in total T, CD+4T and the ratio of CD+4/CD+8 between the two groups.Conclusion The patients with HBeAg-negative had a lower HBVDNA level than those with HBeAgpositive, which might be related to the relatively stronger cellular immune status in patients with HBeAg-negative chronic hepatitis B.
- chronic hepatitis B /
- cellular immunity /
- HBVDNA level /
- T lymphocyte subsets

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参考文献(6)

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